滇重楼总皂苷对涎腺腺样囊性癌ACC-83细胞增殖抑制及其机制的研究

doi:10.7518/hxkq.2017.03.016

华西口腔医学杂志 ›› 2017, Vol. 35 ›› Issue (3): 317-321.doi: 10.7518/hxkq.2017.03.016

滇重楼总皂苷对涎腺腺样囊性癌ACC-83细胞增殖抑制及其机制的研究

何秋敏¹(), 许彪¹(), 王卫红¹, 包崇云², 胡少伟¹

1.昆明医科大学附属口腔医院口腔颌面外科,昆明 650106
2.口腔疾病研究国家重点实验室,国家口腔疾病临床研究中心,四川大学华西口腔医院,成都 610041

收稿日期:2017-02-18 修回日期:2017-04-12 出版日期:2017-06-05 发布日期:2017-06-01
作者简介:
何秋敏,硕士,E-mail：1165679243@qq.com
基金资助:
口腔疾病研究国家重点实验室开放课题（SKLOD2015OF10）;云南省医疗卫生单位内设研究机构科研项目（2016NS115）

Inhibitory effect and underlying mechanism of total saponins from Paris polyphylla var. yunnanensis on the prolifera-tion of salivary adenoid cystic carcinoma ACC-83 cells

Qiumin He¹(), Biao Xu¹(), Weihong Wang¹, Chongyun Bao², Shao-wei Hu¹

1. Dept. of Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital of Kunming Medical University, Kunming 650106, China
2. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

Received:2017-02-18 Revised:2017-04-12 Online:2017-06-05 Published:2017-06-01

摘要/Abstract

摘要：

目的探讨滇重楼总皂苷对涎腺腺样囊性癌ACC-83细胞的增殖抑制作用及其相关机制,为滇重楼治疗涎腺腺样囊性癌提供理论依据。方法通过体外细胞培养,采用CCK-8法检测不同药物质量浓度（5、10、20、40、60、80、100 μg·mL^-1）的滇重楼总皂苷对ACC-83细胞的增殖抑制效应,采用流式细胞仪检测不同药物质量浓度（25、50、100 μg·mL^-1）的滇重楼总皂苷对ACC-83细胞的凋亡率,Western blot及逆转录聚合酶链反应法检测巨噬细胞游走抑制因子（MIF）和CD74的表达。结果滇重楼总皂苷可促进ACC-83细胞凋亡,并呈剂量-效应关系。ACC-83细胞中存在MIF和CD74的表达,滇重楼总皂苷可抑制MIF和CD74的表达。结论滇重楼总皂苷对ACC-83细胞的生长有明显抑制作用,可以抑制MIF及CD74的表达,诱导细胞凋亡,为其临床应用提供了依据。

关键词: 滇重楼总皂苷, 涎腺腺样囊性癌, 巨噬细胞游走抑制因子

Abstract:

Objective To investigate the inhibitory effect and underlying mechanism of total saponins from Paris polyphylla var. yunnanensis on the proliferation of salivary adenoid cystic carcinoma ACC-83 cells. Methods In vitro cell culture was performed. The proliferation of ACC-83 cells treated with different concentrations (5, 10, 20, 40, 60, 80, 100 μg·mL^-1) of total saponins from Paris polyphylla var. yunnanensis was observed using CCK-8 assay. Meanwhile, the apoptosis of ACC-83 cells treated with different concentrations (25, 50, 100 μg·mL^-1) of the total saponins was observed using flow cytometry. The expression levels of macrophage migration inhibitory factor (MIF) and CD74 were measured using Western blot and reverse transcription-polymerase chain reaction. Results The total saponins from Paris polyphylla var. yunnanensis induced apoptosis and expressed dose-effect relationship. ACC-83 cells expressed MIF and CD74, and the total saponins suppressed MIF and CD74 expression in ACC-83 cells. Conclusion The total saponins from Paris polyphylla var. yunnanensis can significantly inhibit the proliferation, suppress MIF and CD74 expression, and promote apoptosis in ACC-83 cells. This study provides a theoretical basis for the treatment of salivary adenoid cystic carcinoma using Paris polyphylla var. yunnanensis.

Key words: total saponins from Paris polyphylla var. yunnanensis, salivary adenoid cystic carcinoma, macro-phage migration inhibitory factor

中图分类号:

R 730.52

何秋敏, 许彪, 王卫红, 包崇云, 胡少伟. 滇重楼总皂苷对涎腺腺样囊性癌ACC-83细胞增殖抑制及其机制的研究[J]. 华西口腔医学杂志, 2017, 35(3): 317-321.

Qiumin He, Biao Xu, Weihong Wang, Chongyun Bao, Shao-wei Hu. Inhibitory effect and underlying mechanism of total saponins from Paris polyphylla var. yunnanensis on the prolifera-tion of salivary adenoid cystic carcinoma ACC-83 cells[J]. West China Journal of Stomatology, 2017, 35(3): 317-321.

图/表 4

参考文献 17

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药物质量浓度/（μg·mL^-1）	OD值	抑制率/%
阴性对照	1.646 33±0.011 2	-
5	1.546 11±0.026 6	6.721±2.351
10	1.438 67±0.047 7	14.264±2.267
20	1.174 73±0.004 4	30.807±6.279
40	0.979 28±0.043 1	43.792±3.343
60	0.697 69±0.021 6	62.295±2.801
80	0.535 33±0.021 2	73.429±0.402
100	0.318 42±0.003 6	87.407±2.333

滇重楼总皂苷对涎腺腺样囊性癌ACC-83细胞增殖抑制及其机制的研究

Inhibitory effect and underlying mechanism of total saponins from Paris polyphylla var. yunnanensis on the prolifera-tion of salivary adenoid cystic carcinoma ACC-83 cells

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