华西口腔医学杂志

• 基础研究 • 上一篇    下一篇

高致龋性变异链球菌临床分离株的初步筛选

王成龙1  刘佼佼2  苏东华1  储冰峰1  李少华3  夏伟3  罗燕萍4  杨继勇4  丁红梅3  赵强3  邓斌1  席庆1  徐娟1  邵宁生3   

  1. 1.中国人民解放军总医院口腔科,北京 100853;2.沈阳军区总医院口腔科,沈阳 110840;3.军事医学科学院基础医学研究所,北京 100850;4.中国人民解放军总医院微生物科,北京 100853
  • 出版日期:2013-04-01 发布日期:2013-04-01
  • 通讯作者: 王成龙,Tel: 010-66937964
  • 作者简介:王成龙(1966—),男,安徽人,副教授,博士
  • 基金资助:

    国家自然科学基金资助项目(30772426,81041028)

Preliminary screen of high cariogenicity Streptococcus mutans strains isolated from clinical specimens

Wang Chenglong1, Liu Jiaojiao2, Su Donghua1, Chu Bingfeng1, Li Shaohua3, Xia Wei3, Luo Yanping4, Yang Jiyong4, Ding Hongmei3, Zhao Qiang3, Deng Bin1, Xi Qing1, Xu Juan1, Shao Ningsheng3   

  1. 1. Dept. of Stomatology, Chinese PLA General Hospital, Beijing 100853, China; 2. Dept. of Stomatology, Chinese PLA Shenyang General Hospital, Shenyang 110840, China; 3. Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China; 4. Dept. of Microbiology, Chinese PLA General Hospital, Beijing 100853, China
  • Online:2013-04-01 Published:2013-04-01

摘要:

目的  初步筛选高致龋性变异链球菌临床分离株。方法  通过检测41株变异链球菌临床分离株的产酸能力、耐酸能力、黏附能力和合成细胞外多糖能力,初步筛选出高致龋性变异链球菌临床分离株。结果  不同的变异链球菌临床分离株体外致龋能力不同,其中3株产酸能力、耐酸能力、黏附能力和合成细胞外多糖能力均较高,提示可能为高致龋性变异链球菌临床分离株,另外3株产酸能力、耐酸能力、黏附能力和合成细胞外多糖能力均较低,提示可能为低致龋性变异链球菌临床分离株。结论  通过筛选可能获得了高致龋性变异链球菌临床分离株。

关键词: 变异链球菌, 产酸, 耐酸, 黏附, 细胞外多糖, 筛选

Abstract:

Objective  To screen of high cariogenicity Streptococcus mutan( (S. mutans) strains isolated from clinical specimens preliminary. Methods  Acidogenicity, aciduricity, extracellular polysaccharide production and adhesion of 41 strains of S. mutans isolated from clinical specimens were investigated to screen high cariogenicity S. mutans strains. Results There were different cariogenicity among 41 strains of S. mutans, in which 3 strains of S. mutans had all high ability to produce extracellular polysaccharide, adhere to the saliva-coated hydroxyapatite, produce acid and tolerate acid, indicated there were 3 strains with high cariogenicity S. mutans strains isolated from clinical specimens. Another 3 strains of S. mutans with all low ability to produce extracellular polysaccharide, adhere to the saliva-coated hydroxyapa- tite, produce acid and tolerate acid indicated they were low cariogenicity S. mutans strains isolated from clinical specimens. Conclusion  We may have obtained high cariogenicity S. mutans strains isolated from clinical specimens.

Key words: Streptococcus mutans, acidogenicity, aciduricity, adhesion, extracellular polysaccharide, screen