华西口腔医学杂志

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渗氮类金刚石薄膜应用于纯钛后的细菌黏附变化

尹路 肖云   

  1. 厦门市口腔医院修复科, 厦门361003
  • 收稿日期:2011-10-25 修回日期:2011-10-25 出版日期:2011-10-20 发布日期:2011-10-20
  • 通讯作者: 尹路,Tel:0592-2669535
  • 作者简介:尹路(1979—),男,福建人,主治医师,博士
  • 基金资助:

    福建省自然科学基金青年创新基金资助项目(2009D013);厦门市杰出青年创新基金资助项目(3502Z20105010)

The change of bacterial adhesion during deposition nitrogen-diamond like carbon coating on pure titanium

Yin Lu, Xiao Yun.   

  1. Dept. of Prosthodontics, Xiamen Stomatological Hospital, Xiamen 361003, China
  • Received:2011-10-25 Revised:2011-10-25 Online:2011-10-20 Published:2011-10-20
  • Contact: Yin Lu,Tel:0592-2669535
  • About author:

摘要:

目的观察纯钛试件应用等离子镀膜法镀制纳米渗氮类金刚石(N-DLC)薄膜后的细菌黏附变化,以期对临床应用有所指导。方法采用等离子镀膜机,在纯钛试件表面沉积N-DLC薄膜、TiN薄膜、阳极氧化膜以及空白对照,然后将其黏附于树脂基托表面,实际使用6个月后,扫描电子显微镜观察白色假丝酵母菌黏附试件情况;粗糙度检测仪对比检测试件粗糙度变化。结果白色假丝酵母菌在N-DLC膜表面附着数量比其他组试件明显少(P<0.05),且菌体生长不良,处于抑制状态;阳极氧化膜和空白对照试件表面黏附菌量最多,且菌体生长旺盛。N-DLC膜表面粗糙度在戴用前后变化最小(P<0.05)。结论纯钛表面镀制N-DLC膜在口腔实际应用过程中可以明显降低白色假丝酵母菌的黏附量,从而降低罹患义齿性口炎的风险。

关键词: 纯钛, 表面改性, 粗糙度

Abstract:

Objective The aim of this study was to observe the change of bacterial adhesion on pure titanium coated with nitrogen-diamond like carbon(N-DLC) films and to guide the clinical application. Methods N-DLC was deposited on titanium using ion plating machine, TiN film, anodic oxide film and non-deposition were used as control, then made specimens adhering on the surface of resin denture base for 6 months. The adhesion of Saccharomyces albicans on the titanium surface was observed using scanning electron microscope, and the roughness was tested by roughness detector. Results The number of Saccharomyces albicans adhering on diamond-like carbon film was significantly less than on the other groups(P<0.05), and the growth of bacterial cell was inhibited and in a poor state. The largest number of adhesion and cell strains grew well on anodic oxide film group and non-deposition control group. The change of surface roughness of N-DLC film was less than other group(P<0.05). Conclusion Pure titanium coated with N-DLC film reduced the adhesion of Saccharomyces albicans after clinical application, thereby reduced the risk of denture stomatitis.

Key words: titanium, surface modification, roughness