Cadherin switching induced by P120-catenin can promote the migration and invasion of oral squamous cell cancer cells

doi:10.7518/hxkq.2017.02.014

Abstract

Abstract:

Objective The main goal is to investigate the role of P120-catenin (P120^ctn) in cadherin switching, as well as migration and invasion, of oral squamous cell cancer (OSCC) cells. Methods The plasmid pGFP-V-RS-P120^ctn shRNA was used to transfect TSCCA cells and significantly reduce the expression of P120^ctn in these cells. Real-time fluorescent quantita-tive polymerase chain reaction and Western blot were conducted to determine the mRNA and protein expression levels of P120^ctn, E-cadherin (E-cad), and N-cadherin (N-cad). By contrast, the Transwell cell invasion and cell migration assay was used to determine the invasion and migration capacities before and after the transfection. Results After the plasmid pGFP-V-RS-P120^ctn shRNA was transfected into the TSCCA cells, we found that as the P120^ctn expression significantly decreased, E-cad mRNA and protein expression decreased significantly. Moreover, N-cad mRNA and protein expression increased sig-nificantly (P<0.05). Lastly, the cell migration and invasion capacities were augmented significantly (P<0.05). Conclusion In OSCC cells, P120^ctn may be involved in cadherin switching and promote metastasis and invasion.

Key words: P120-catenin, cadherin switching, oral squamous cell cancer

CLC Number:

R739.8

Zhong Chen, Mei Zhang, Yong Xu. Cadherin switching induced by P120-catenin can promote the migration and invasion of oral squamous cell cancer cells[J]. West China Journal of Stomatology, 2017, 35(2): 183-186.

Figures/Tables 3

References 13

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