West China Journal of Stomatology

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Regulation of XW630 on TGF-B1and CollagenÑ Gene Expression in Osteoblasts

Fei Wei, Wang Dazhang, Zheng Hu, et al   

  • Received:2001-12-25 Revised:2001-12-25 Online:2001-12-20 Published:2001-12-20

Abstract:

Objective:The purpose of this partof studywas to investigate the mechanism of a newanti-osteoporotic drug, XW630 on pro- moting osteogenic activity.Methods:Total RNAwere isolated from osteoblasts of the mice after the reactionwith XW630 in 24 and 72 hours, that were hybridized with the extracted and digoxigenin-labeled TGF-B1and the collagenÑcDNA probes.Results: XW630 significantly promoted TGF-B1and collagenÑgene expression in osteoblasts, and this drug was superior than other two kindsof estrogen in promoting osteogenic activity (P<0.05).Conclusion:XW630 promote osteogenic activity of osteoblasts, probably by enhancing the mRNA expression of TGF-B1and collagenÑ.

Key words: osteoblast, TGFβ-1, collagenⅠ, geneexpression