Abstract:

Objective To investigate the expression and correlation of mast cell, Clusterin/apoJ and transforming growth factor-β（TGF-β） in the different stages of human dermal hemangioma. Methods Immunohistochemical stain technique（SABC） and toluidine blue（TB） stain technique were respectively used to detect the expression level of Clusterin/apoJ and TGF-β and the number of mast cells in the different stages of human dermal hemangioma. Results There was remarkable statistical difference between the advanced stage of proliferative hemangioma and the other stages of proliferative hemangioma in the number of mast cell（P<0.01）. There was also remarkable statistical difference between the early stage of involutional hemangioma and the other stages of involutional hemangioma in the number of mast cell（P<0.01）. The expression of Clusterin/apoJ and TGF-β in the advanced stage of proliferative hemangioma was significantly higher than the other stages in proliferative hemangioma（P<0.01）. The expression of Clusterin/apoJ and TGF -β in the early stage of involutional hemangioma was significantly higher than the other stages in involutional hemangioma（P<0.01）. There was a significantly positive correlation between Clusterin/apoJ and TGF-β in the different stages of human dermal hemangioma（P<0.01）. The expression level of Clusterin/apoJ and TGF-β was positively correlated with the number of mast cell in the different stages of human dermal hemangioma（P <0.01）.Conclusion Mast cell may play a promotive role of apoptosis during the spontaneous regulate the expression of Clusterin/apoJ and promote the spontaneous involution of human dermal hemangioma.

Key words: hemangioma, vascular endothelial cells, mast cell, Clusterin/apoJ, transforming growth factor-β