Abstract:

Objective　To evaluated the cytotoxicity of a newnano-hydroxyapatite (n-HA)root canal sealer.Methods　In this study, the cytotoxicity was evaluated by the method of MTT assayin vitro, and culture medium F12 as control, three concentra- tions of the soaking material cultured with mouse osteoblast separately, to test the cell relative growth rate(RGR) of every group. Results　The toxicity graduation of the n-HA root canal sealer tends to 0 with the culture time increasing. The cell survival rate of n-HA root canal sealer showed high relatively. The OD value of cell was similar for the negative control and the extracts(P> 0·05).Conclusion　The result indicated that n-HA root canal sealer was compatible with the testcells.

Key words: nano-hydroxyapatite, root canal sealer, cytotoxicity