Abstract:

Objective　To detectPorphyromonas gingivalis(P.gingivalis) in buccal epithelial cells and subgingival plaque from periodontally healthy subjects and patients with chronic periodontitis.Methods　40 subjects were included in the healthy group and 39 subjectswere included in the diseased group in this study. Cells and subgingival plaque sampleswere collected. The extracted DNAwas amplifiedwith universal primers andP.gingivalisspecies-specific primer.Results　P.gingivaliswas detect- ed in 37.5% of subgingival plaque samples and 32.5% of buccal mucosa samples in the healthy subjects, but 69.23% of subgin- gival plaque samples and 46.15% of buccal mucosa samples in the periodontitis group. Highly statistically significant differences were observed between healthy and periodontitis groups in the detections ofP.gingivalisof subgingival plaque samples.Conclu- sion　P.gingivalismay be one of oral flora because it can be detected in the healthy population and not lead to destruction of supporting structures of the teeth.

Key words: Porphyromonas gingivalis, buccal mucosa, chronic periodontitis