Abstract:

Objective　To observe human tumor necrosis factor-α(hTNF-α) expression and secreting level of human embryo myoblasts transfected by hTNF-αgene.Methods　Human embryo myoblasts were transfected with shuttle plasmid pSV23SHTNF containing hTNF-αgene by cationic liposomes DOSPER. The control group was only given equivalent liposomes except plasmid. After culturing for 24, 48, 72 and 96 hours, hTNF-αexpression level of human embryo myoblastswas observed with immunocyto- chemistry staining, and hTNF-αsecreting of human embryo myoblasts was analyzed by ELISA.Results　After transfected by hT- NF-αgene for 24 , 48, 72 and 96 hours, the human embryomyoblasts displayed significant secretion of hTNF-αin the cultural su- pernatant(P<0.05), and overexprsssion in cytoplasma and cell membrane.Conclusion　Transfection of hTNF-αgene to human myoblasts made myoblasts secrete high concentration of hTNF-α, implying it is feasible that transfecting muscle cells surrounding tongue carcinoma lesion with hTNF-αgene can prevent tongue carcinoma from intruding into deeper muscle tissue.

Key words: human tumor necrosis factor-α, gene transfection, human embryo myoblasts, gene expression