Abstract:

Objective　Quantitative study of the effect of anti-human VEGF mAb E11to VEGF level in serum of nude mice transplanted buccal carcinoma.Methods　E11was administered into BALB/c nu/nu mice which were transplanted human buccal carcinoma. The saline was administrated as negative control. Mice were killed at 18 days. The VEGF level in serum of mice was determined by improved indirect ELSIA.Results　Comparedwiththe VEGF level in serum ofmice in saline group, itwas dramat- ically decrease in E11group. The VEGF level in serum of mice treated E11by subcutaneouswas lowest and only reached (1.17± 0.13)μg/L.Conclusion　It demonstrated that the anti-human VEGF mAb could reduce the VEGF level in serum by binding VEGF, and block its biological activity. It indicates that VEGF in serum of malignant tumor patient is a new tumor marker.

Key words: vascular endothelial growth factor, monoclonal antibody, enzyme-linked immunosorbent assay