Abstract:

Objective:The purpose of this study was to evaluate the effects of the exogenous phosphatase and tensin homology deleted on chromosome 10 (PTEN) gene on in vitro growth of the highly metastatic mucoepidermoid carcinoma cell line M3SP2.Methods: The growth of the exogenous PTEN transfected mucoepidermoid carcinoma cells M3SP2-PTEN gene was studied by analyzing cell growth curves, mitosis index and clone formation efficiency and compared with its parental cell line M3SP2and the vector pBabe- puro-transfected cell line M3SP2-pBp.Results:The doubling time (h) ofM3SP2, M3SP2-pBp andM3SP2-PTENwere 24.50, 24. 76 and 31.74; the mitosis index(j) were 53.0?6.20, 49.0?5.24 and 16.2?3.2; the clone formation efficiency (%) were 37.37, 35.01 and 10.40, respectively. The M3SP2-PTEN cells also revealed 57.05%~71.46% inhibition of growth from day 3 to7 and 65%~72% inhibition of clone formation compared with the parental cells.Conclusion:These data provide evidence that the exogenous wild-type PTEN have remarkably inhibitory effects on in vitro proliferation of the highly metastatic mucoepidermoid carcinoma cell line M3SP2.

Key words: phosphatase and tensin homology deleted on chromosome 10, mucoepidermoid carcinoma, gene transfection