Abstract:

The purpose of this study is to investigate the synergistic effects of human tumor necrosis factor-α (hTNF-α) trans2 fection and interferon-γ ( IFN-γ) on the growth of tongue carcinoma cells.Methods : The cultured Tca8113 tongue carcinoma cells was divided into 2 groups , one group was transferred with hTNF-αgene. Each of the 2 groups was then divided into 5 subgroups , and the subgroups were added IFN-γuntil the final IFN-γconcentrations respectively were 0 , 10 , 100 , and 1000 UPml. After cul2 turing for 48 hours , the survival rates of the all groups of cells were assayed byMTT enzymatic labeling technique , and the expres2 sion of hTNF-αin Tca8113 cells was observed with immunocytochemistry. Results : IFN-γdid not affect the growth of Tca8113 cells without hTNF-α, however , the transfection of hTNF-αwith the above different concentrations of IFN-γ synergistically inhibited the growth of Tca8113 cells , the concentrations of IFN-γwere positively correlated with the inhibition effects ( r = 0. 733 , P < 0. 01) , the transferred Tca8113 cells displayed remarkable overexpression of hTNF-α, compared with the non-transferred. Conclusion : IFN-γcan enhance the inhibition effects of hTNF-αtransfection on the tongue carcinoma cells.

Key words: human tumor necrosis factor-α, interferon-γ, gene transfection, tongue carcinoma