West China Journal of Stomatology

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A Study of Analyzing Short-chain Fat Acid by Gradient Reversed-phase High-perfermance Liquid Chromatography

Zhang Ping, Ye Liming, Xiao Xiaorong, et al   

  1. Key Laboratory,College of Stomatology,West China University of Medical Scienses
  • Received:2001-10-25 Revised:2001-10-25 Online:2001-10-20 Published:2001-10-20


Objective:The objective of this study is to obtain the optimized separating conditions of formic acid, acetic acid, propionic acid, butyric acid, valeric acid, lactic acid, succinic acid from bacteria using reversed-phase high performance liquid chromato- graph (RP-HPLC), and to set up a reliable progress in identifying the end-products of oral bacteria.Methods: The conditions of separating seven short-chain fat acidswere optimized using the method of gradient RP-HPLC.Results: The study showed that the optimal chromatographic parameters were: 110 mlPmin and 210 mlPmin of flow rates, and the wavelength of ultraviolet detection was 217 nm. The composition of eluent: the ratio of phosphate buffer(pH218) tomethanol is95B5 (vPv), gradient5%~50%. The liner gradientwash will be performed in 10 minutes at room temperature. The average recovery rate was 92%, and RSDwas 118%.Conclusion:The results of this paperwill be helpful to determine acid composition of end products of oral bacteria.

Key words: reversed-phase high-perfermance liquid chromatography, short-chain fat acid, oral bacteria