Effects of cell area on single odontoblast polarization and differentiation via microarray technology

doi:10.7518/hxkq.2025.2024392

West China Journal of Stomatology ›› 2025, Vol. 43 ›› Issue (2): 183-189.doi: 10.7518/hxkq.2025.2024392

• Basic Research • Previous Articles Next Articles

Effects of cell area on single odontoblast polarization and differentiation via microarray technology

Li Huen¹^,²(), Yu Nianzuo³^,⁴(), Li Xiheng¹^,², Tang Xiaoduo²^,³, Sun Yalu¹^,², Si Chao¹^,², Zhang Junhu³^,⁴(), Chang Bei¹^,²()

^1.Dept. of Pediatric Dentistry, Hospital of Stomatology, Jilin University, Changchun 130021, China
^2.Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun 130021, China
^3.Institute of Translational Medicine, The First Hospital of Jilin University, Changchun 130021, China
^4.College of Chemistry, Jilin University, State Key Laboratory of Supramolecular Structure and Materials, Changchun 130021, China

Received:2024-10-24 Revised:2025-02-17 Online:2025-04-01 Published:2025-03-25
Contact: Zhang Junhu,Chang Bei E-mail:lhe22@mails.jlu.edu.cn;ynz@jlu.edu.cn;zjh@jlu.edu.cn;bchang@jlu.edu.cn
Supported by:
Natural Science Foundation of China(82101075);Young Elite Scientists Sponsorship Program by China Association for Science and Technology(YESS20210407);China Postdoctoral Science Foundation(2022M711301);Bethune Project of Jilin University(419161923025)

Abstract

Abstract:

Objective This study aimed to explore the impact of cell spreading area on odontoblast polarization and differentiation using micropatterned surfaces ge-nerated by photolithography. Methods Micropatterned surfaces with differential adhesive properties were prepared using polyethylene glycol diacrylate (PEGDA)-ba-sed photolithography. Human dental pulp stem cells (hD-PSCs) were isolated into single cells and cultured on micropatterned surfaces with areas of 1 800, 2 700, and 3 600 μm². Immunofluorescence staining was used to observe cell morphology and analyze the relocating of the golgi apparatus and nucleus. Alkaline phosphatase staining was preformed to examine odontogenic differentiation. Results The hDPSCs were successfully isolated and cultured on micropatterned surfaces mimicking the morphology of polarized odontoblasts. Phalloidin staining confirmed that the isolated hDPSCs successfully recapitulated the morphology of predesigned micropatterns. Immunofluorescence staining showed that the polarization and differentiation levels of the hDPSCs with a 3600 μm² area were significantly higher than those with 1 800 and 2 700 μm² areas (P<0.05). Conclusion The polarization and differentiation of single hDPSCs increased with the cell areas on micropatterned surfaces.

Key words: cell polarization, micropatterning, odontoblast, cell differentiation

CLC Number:

R781.1

Li Huen, Yu Nianzuo, Li Xiheng, Tang Xiaoduo, Sun Yalu, Si Chao, Zhang Junhu, Chang Bei. Effects of cell area on single odontoblast polarization and differentiation via microarray technology[J]. West China Journal of Stomatology, 2025, 43(2): 183-189.

Figures/Tables 6

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Effects of cell area on single odontoblast polarization and differentiation via microarray technology

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