Effect of micro/nanoscaled Ti phosphate/Ti oxide hybrid coating on the osseointegration of Ti implants

doi:10.7518/hxkq.2021.05.006

Abstract

Abstract: Objective

This study was performed to fabricate a bionic coating with titanium (Ti) phosphate to promote the osseointegration of Ti substrate implants.

Methods

Phosphorylated micro/nanocoating was prepared on the surface of pure titanium (i.e., TiP-Ti) by hydrothermal process under special pressure, and the untreated smooth pure titanium (cp-Ti) was selected as the control. To evaluate the characteristics of the coating surface, scanning electron microscopy, X-ray diffraction, atomic force microscopy, and contact-angle measurement were performed. In addition, the effects of TiP-Ti on the proliferation, adhesion, and differentiation of rat bone marrow mesenchymal stem cells (BMSCs) were investigated by using in vitro cytology. Finally, TiP-Ti implants were implanted into the rat tibia, and the effect of TiP-Ti on the osseointegration in the host was evaluated after 12 weeks.

Results

The TiP-Ti surface presented a bionic structure with coexisting nanoscale 3D spatial structure and microscale pores. In vitro experiments showed that the BMSCs had enhanced adhesion, proliferation, and osteogenic differentiation on the TiP-Ti surface. Furthermore, in vivo, TiP-Ti showed considerably stronger osseointegration compared with pure titanium, and the ultimate shear strength and maximum pushing force were significantly improved.

Conclusion

A bionic structure with TiP-Ti micro/nanoscale coating was successfully fabricated, indicating a promising method for modifying the surface of implants.

Key words: Ti phosphate, Ti oxide, osseointegration, micro/nanoscale morphology, hierarchical structure

CLC Number:

R 783.4

Zhang Jie, Zhu Songsong, Jiang Nan.. Effect of micro/nanoscaled Ti phosphate/Ti oxide hybrid coating on the osseointegration of Ti implants[J]. West China Journal of Stomatology, 2021, 39(5): 531-539.

Figures/Tables 13

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项目	cp-Ti	TiP-Ti	t值	P值
RMS/nm	6.35±2.4	58.65±8.2	3.640	0.010
Z范围/nm	182.83±22.45	569.04±50.43	2.450	0.020
表面积差/%	4.23±0.88	32.54±4.56	3.640	0.010

项目	cp-Ti	TiP-Ti	t值	P值
最大推出力/N	34.5±3.5	182.7±10.2	3.250	0.010
极限抗剪切强度/（N·mm^-2）	9.63±0.25	22.87±0.55	4.297	0.020

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