The construction and expression of Saccharomyces albicans pACT1-GFP plasmids

doi:10.3969/j.issn.1000-1182.2012.02.002

West China Journal of Stomatology

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The construction and expression of Saccharomyces albicans pACT1-GFP plasmids

Sun Jing, Jia Fen, Xia Minghui, Qian Hua, Dong Hongnan, Qi Qingguo

School of Stomatology of Shandong University, Key Laboratory of Oral Biomedicine of Shandong Province, Jinan 250012, China

Received:2012-04-25 Revised:2012-04-25 Online:2012-04-01 Published:2012-04-01
Contact: Qi Qingguo，Tel：0531-88382213
About author:Sun Jing（1983—），女，山东人，博士

Abstract

Abstract:

Objective To construct strains containing green fluorescent protein（GFP） to study gene regulation in Saccharomyces albicans cells during the infection process. Methods pACT1-GFP was constructed, and Saccharomyces albicans CAI4 was transformed. The expression of GFP in yeast and hyphal compartments was observed with microscopy. Results 99% of Saccharomyces albicans cells containing pACT1-GFP fusion displayed significant fluorescence levels both in the yeast and hyphal compartments. The fluorescence intensity in two compartments had no obvious difference. Conclusion pACT1-GFP can be expressed stably in the yeast cells.

Key words: Saccharomyces albicans, green fluorescent protein, plasmid

Sun Jing, Jia Fen, Xia Minghui, Qian Hua, Dong Hongnan, Qi Qingguo. The construction and expression of Saccharomyces albicans pACT1-GFP plasmids[J]. West China Journal of Stomatology, doi: 10.3969/j.issn.1000-1182.2012.02.002.

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The construction and expression of Saccharomyces albicans pACT1-GFP plasmids

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