变形链球菌F-ATPase亚基基因uncEBF基因多态性的研究

华西口腔医学杂志

变形链球菌F-ATPase亚基基因uncEBF基因多态性的研究

杨德琴1, 2, 刘天佳1, 付春华1, 亓庆国3, 庄姮1, 李颂4

1.口腔生物医学工程教育部重点实验室, 四川大学, 四川成都610041; 2.遵义医学院附属口腔医院口腔内科, 贵州遵义563003; 3.山东大学口腔医院牙体牙髓科, 山东济南250012; 4.安徽医科大学口腔医院牙体牙髓科, 安徽合肥230000

收稿日期:2007-06-25 修回日期:2007-06-25 出版日期:2007-06-20 发布日期:2007-06-20
通讯作者: 刘天佳，Tel：028- 85501439
作者简介: 杨德琴（1972-），女，广西人，副教授，博士
基金资助:
国家自然科学基金资助项目（30171013）；贵州省自然科学基金资助项目[黔科合计（2004）3055]；贵州省优秀科技人才省长专项基金资助项目[（2005）244]

Genetic Diver sity of F- ATPase Subunits Gene uncEBF Amplified from Streptococcus mutans Clinical Isolates

YANG De- qin1，2, LIU Tian- jia1, FU Chun- hua1, QI Qing- guo3, ZHUANG Heng1, LI Song4

1. Key. Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China; 2. Dept. of Oral Medicine, Affiliated Hospital of Stomatology, Zunyi Medical College, Zunyi 563003, China; 3. Dept. of Operative Dentistry and Endodontics, College of Stomatology, Shandong University, Jinan 250012, China; 4. Dept. of Operative Dentistry and Endodontics, College of Stomatology, Anhui University of Medical Science, Hefei 230000, China

Received:2007-06-25 Revised:2007-06-25 Online:2007-06-20 Published:2007-06-20
Contact: LIU Tian- jia，Tel：028- 85501439

摘要/Abstract

摘要：

目的研究变形链球菌临床分离株耐酸因子F- ATPase亚基c、a、b联合基因uncEBF的遗传多态性，并探讨基因多态与细菌耐酸力的关系。方法选取18株变形链球菌高耐酸株、20株低耐酸株，以特异引物从细菌组DNA扩增uncEBF，行限制性内切酶长度多态性分析（RFLP）和测序比较。结果不同限制性内切酶RFLP产生不同的基因型，测序证实了导致多态出现的基因变异；其中内切酶AluⅠ产生的A、B基因型在不同耐酸力菌株的分布不同（P<0.05），高耐酸性菌株中A型基因uncEBF的检出高于低耐酸力菌株。结论变形链球菌F- ATPase亚基联合基因uncEBF具有明显基因多态性，酸性环境下生存力强的菌株可能出现基因的适应性变异，可认为不同基因型uncEBF分布与耐酸力不同相关。

关键词: 变形链球菌, 耐酸性, 基因多态性

Abstract:

Objective The purpose of this research was to study the genetic diversity of F- ATPase subunit gene uncEBF derived from Streptococcus mutans（S.mutans）clinical isolates, furthermore to investigate the relationship between the genetic diversity of F- ATPase and S.mutans aciduric ability. Methods 38 S.mutans strains included 18 high acid tolerance strains and 20 low acid tolerance strains. Gene uncEBF of these isolates were amplified with specific primers from S.mutans genomic DNA, and the PCR products were analyzed by RFLP and sequenced. SPSS 11.0 statistic software assayed the results. Results It was testified that two genotypes A and B of PCR- RFLP were revealed when digested with AluⅠand DdeⅠdigested fragments of uncEBF displayed two different patterns C and D. Fisher exact two- tail test showed that the distributions of A and B genotype strains with different acidurance were different（P<0.05）, and the proportion of A genotype strains from high acidurance group was higher than that from low acidurance one. Some of these amplified uncEBF genes from different genotype were sequenced and testified that there existed variation of AluⅠand DdeⅠrecognized sites. Conclusion This study indicated that uncEBF gene of S.mutans F- ATPase obviously exhibited genetic diversity.

Key words: Streptococcus mutans, aciduric, genetic diversity

杨德琴刘天佳付春华亓庆国庄姮李颂. 变形链球菌F-ATPase亚基基因uncEBF基因多态性的研究[J]. 华西口腔医学杂志.

YANG De- qin1，2, LIU Tian- jia1, FU Chun- hua1, QI Qing- guo3, ZHUANG Heng1, LI Song4. Genetic Diver sity of F- ATPase Subunits Gene uncEBF Amplified from Streptococcus mutans Clinical Isolates[J]. West China Journal of Stomatology.

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