华西口腔医学杂志

• 临床研究 • 上一篇    下一篇

纳米羟磷灰石对玻璃离子黏固剂的性能影响

穆亚冰1, 臧光祥2, 孙宏晨2, 王成坤1   

  1. 1.吉林大学口腔医院牙体牙髓病科; 2.病理科, 吉林长春130041
  • 收稿日期:2007-12-25 修回日期:2007-12-25 出版日期:2007-12-20 发布日期:2007-12-20
  • 通讯作者: 孙宏晨,Tel:0431- 88796010
  • 作者简介:穆亚冰( 1977-),女,河南人,主治医师,博士

Effect of nano- hydroxyapatite to glass ionomer cement

MU Ya- bing1, ZANG Guang- xiang2, SUN Hong- chen2, WANG Cheng- kun1   

  1. 1. Dept. of Conservative Dentistry, School of Stomatology, Jilin University, Changchun 130041, China;2. Dept. of Pathology, School of Stomatology, Jilin University, Changchun 130041, China
  • Received:2007-12-25 Revised:2007-12-25 Online:2007-12-20 Published:2007-12-20
  • Contact: SUN Hong- chen,Tel:0431- 88796010

摘要:

目的探讨添加纳米羟磷灰石( nano- HAP)的玻璃离子黏固剂( GIC)的力学性能、充填后的材料- 牙本质界面渗漏状况和矿化性能。方法将nano- HAP按8%的质量分数比均匀混入GIC粉末形成复合材料( 实验组材料),单纯GIC为对照组材料。采用两种材料分别制备20个柱状样本,测定其三点弯曲强度和压缩强度。选用120颗完整的离体牙,在颊面制备Ⅴ类洞,分别采用两种材料充填后放入质量分数1%若丹明- B- 异硫氰酸盐溶液中染色24 h,在激光共聚焦显微镜下观察充填界面的渗漏现象。在健康犬磨牙上制备Ⅴ类洞,分别用两种材料充填,8周后拔除充填牙,在偏振光显微镜下观察材料内部和充填界面的矿化情况。结果添加nano- HAP的GIC复合材料的三点弯曲强度、压缩强度与单纯GIC相比有所提高( P<0.001,P<0.05);添加nano- HAP的GIC复合材料和单纯GIC充填离体牙标本后均出现纳渗漏和微渗漏,但前者出现微渗漏概率较后者低( P=0.004);复合材料充填界面有新形成的羟磷灰石晶体,并沉积形成矿化带,单纯GIC充填界面没有羟磷灰石晶体形成。结论添加nano- HAP的GIC充填牙齿后能形成较紧密的材料- 牙本质界面,并有矿化潜能,可作为窝洞衬里或预防性充填材料应用。

关键词: 玻璃离子黏固剂, 纳米羟磷灰石, 矿化, 微渗漏

Abstract:

Objective To investigate the mechanical character, microleakage and mineralizing potential of nanohydroxyapati(te nano- HAP)- added glass ionomer ceme(nt GIC). Methods 8% nano- HAP were incorporated into GIC as composite, and pure GIC as control. Both types of material were used to make 20 cylinders respectively in order to detect three- point flexural strength and compressive strength. Class Ⅴ cavities were prepared in 120 molars extracted for orthodontic treatment, then were filled by two kinds of material. The microleakage at the composite- dentine interface was observed with confocal laser scanning microscop(e CLSM)after stained with 1% rhodamin- B- isothiocyanate for 24 hours. Class Ⅴ cavities were prepared in the molars of 4 healthy dogs, filled with composite, and the same molars in the other side were filled with GIC as control. The teeth were extracted to observe the mineralizing property with polarimetric microscope in 8 weeks after filling. Results Three- point flexural strength and compressive of nano- HAP- added GIC were increased compared with pure GIC( P<0.001,P<0.05). The nanoleakages and microleakages appeared at the material- dentine interface in the two groups, but there were more microleakages in control group than in experiment grou(p P=0.004). New crystals of hydroxyapatite were formed into a new mineralizing zone at the interface of tooth and nano- HAP- added GIC, while there was no hydroxyapatite crystals formed at the interface of tooth and pure GIC. Conclusion 8% nano- HAP- added GIC can tightly fill tooth and have mineralizing potential, and can be used as liner or filling material for prevention.

Key words: glass ionomer cement, nano- hydroxyapatite, mineralizing, microleakage