关键词: 腺样囊性癌, 同源异型盒基因, 基因芯片

Abstract:

Objective To investigate the differently expressed Homeobox genes between adenoid cystic carcinoma of salivary gland and normal gland tissue, and find out the effect of Homeobox genes on oncogenesis and differentiation of adenoid cystic carcinoma of salivary gland. Methods Six strictly paired specimens including adenoid cystic carcinoma and its surrounding normal gland tissue and two pairs of specimens including cell strain of adenoid cystic carcinoma and its surrounding normal gland tissue were established. Customized Oligo microarray which contains probes of 232 human Homeobox genes was used to analyze and conclude two groups of different genes data. RTPCR technique was used to examine the mRNA expressing level of highly suspected relevant genes of adenoid cystic carcinoma in different specimens. Obvious differently expressed Homeobox genes were found through statistical analyses. Results In tissue specimens Homeobox genes were found 67 up-regulated and 54 down-regulated, and in cell specimens Homeobox genes were found 12 up -regulated and 15 down -regulated. One up -regulated gene and 7 down-regulated genes were found both in tissue and cell specimens, among which EVX1 and PITX1 were the most frequent. RT-PCR showed that there was statistical expressing difference between TGIF, EVX1 and normal gland tissue in ACC-M. Conclusion As the key gene to cellular proliferation and differentiation, Homeobox genes are closely relevant to the oncogenesis of adenoid cystic carcinoma of salivary gland.

Key words: adenoid cystic carcinoma, Homeobox gene, gene microarray