3种铸造合金全冠戴用后Ki67和P53蛋白在牙龈组织中的表达

doi:10.3969/j.issn.1000-1182.2010.05.024

华西口腔医学杂志

3种铸造合金全冠戴用后Ki67和P53蛋白在牙龈组织中的表达

乔广艳1，2 苏俭生1

1.同济大学口腔医院修复科，上海200072； 2.上海市口腔病防治院修复科，上海200001

收稿日期:2010-10-25 修回日期:2010-10-25 出版日期:2010-10-20 发布日期:2010-10-20
通讯作者: 苏俭生，Tel：021-66311629
作者简介:乔广燕（1978—），女，江苏人，主治医师，博士
基金资助:
国家自然科学基金资助项目（30970726）

Expression of Ki67 and P53 proteins in gingival tissue after wearing casting alloy crowns

QIAO Guangyan1，2, SU Jian -sheng1.

1. Dept. of Prosthodontics, School of Stomatology, Tongji University, Shanghai 200072，hina; 2. Dept. of Prosthodontics, Shanghai Stomatology of Dental Clinic, Shanghai 200001, China

Received:2010-10-25 Revised:2010-10-25 Online:2010-10-20 Published:2010-10-20
Contact: SU Jian -sheng，Tel：021-66311629

摘要/Abstract

摘要：

目的研究3种常用铸造合金全冠戴用后Ki67、P53蛋白在牙龈组织中的表达，探讨3种常用牙科铸造合金的生物学性能。方法对犬分别行58%金合金、不含铍的镍铬合金、含铍的镍铬合金铸造全冠修复，建立实验动物模型。应用免疫组化Envision二步法检测戴冠前、戴冠后2周，1、2及3个月时Ki67、P53蛋白在牙龈组织中的表达。结果含铍的镍铬合金与不含铍的镍铬合金铸造全冠戴用后，Ki67、P53蛋白在牙龈组织的表达逐渐升高，戴冠1个月后达到最高，随后逐渐降低；而58%金合金全冠戴用后，Ki67、P53蛋白的阳性表达率与空白对照组无统计学差异，随时间延长也无明显改变。结论镍铬合金全冠戴用后，牙龈组织中Ki67、P53蛋白表达明显，而58%金合金全冠戴用后表达不明显。3种铸造合金材料均未造成长时期的牙龈上皮异常增殖。

关键词: 牙科铸造合金, 牙龈组织, Ki67蛋白, P53蛋白

Abstract:

Objective The aim of the study was to detect the expression of Ki67 and P53 proteins in the gingival issue before and after wearing three kinds of dental casting alloy crowns. Methods The three kinds of dental asting alloys（58% golden alloy, NiCr alloy, NiCr alloy excluding Be） were used to make full crowns for 3 dogs in ach group. Another 3 dogs were taken as control. The immunohistochemistry Envision method was used to measure he expression of Ki67 and P53 proteins in the gingival tissue before wearing the crowns, 2 weeks, 1 month, 2 onths, 3 months after wearing the crowns. Results The expression of Ki67 and P53 proteins in gingival tissue increased month after wearing NiCr alloy and NiCr alloy excluding Be, then decreased gradually. And the expression f Ki67 and P53 proteins in 58% golden alloy group was similar to that in control. No pathological change was observed n all samples. Conclusion Two kinds of NiCr alloy crowns both can increase the expression of Ki67 and 53 proteins in gingival tissue, but 58% golden alloy crowns can not. So compared with NiCr alloy crowns, 58% olden alloy crowns have better biological properties. And none of three kinds of dental alloy crowns may induce ong-term abnormal proliferation in gingival tissue.

Key words: dental casting alloys, gingival tissue, Ki67 protein, P53 protein

乔广艳苏俭生. 3种铸造合金全冠戴用后Ki67和P53蛋白在牙龈组织中的表达[J]. 华西口腔医学杂志, doi: 10.3969/j.issn.1000-1182.2010.05.024.

QIAO Guangyan1，2, SU Jian -sheng1.. Expression of Ki67 and P53 proteins in gingival tissue after wearing casting alloy crowns[J]. West China Journal of Stomatology, doi: 10.3969/j.issn.1000-1182.2010.05.024.

[1]	赵耀童徐刘嘉俊浩志超孟玉坤. 不同热处理对牙科银钯铸造合金显微硬度的影响[J]. 华西口腔医学杂志, 2013, 31(3): 235-236.
[2]	韩帮锋吴平凡潘剑李一李龙江. 鼠双微粒体2蛋白、P53在口腔白斑和鳞癌中的表达及其相关性的研究[J]. 华西口腔医学杂志, 2011, 29(01): 79-82.
[3]	童徐赵耀孟玉坤. 2种石膏基包埋料对铸造精度影响的比较研究[J]. 华西口腔医学杂志, 2009, 27(06): 649-652.
[4]	苏俭生;邓章悦;邵磊;乔广艳;万书健. 铸造合金全冠戴用后颊黏膜上皮细胞DNA损伤的研究[J]. 华西口腔医学杂志, 2006, 24(01): 21-25.
[5]	周红梅,杨娅,周敏,郑晓晖,王智,何志秀,李秉琦. 口腔黏膜组织结构及DNA损伤修复功能的增龄性变化[J]. 华西口腔医学杂志, 2003, 21(03): 177-179.
[6]	杨丕山,赵民,戚向敏,葛少华. 外源性IL-10对炎症牙龈组织中IL-6和ICAM-1表达的影响[J]. 华西口腔医学杂志, 2002, 20(05): 343-345.
[7]	刘斌,司徒镇强,吴军正,陈建元. HMBA对粘液表皮样癌P53蛋白表达和细胞增殖周期的影响[J]. , 1999, 17(01): 0-.
[8]	宋红!710032,王字玲!710032,赵瑞芳!710032,周以钧!710032. 牙周炎龈组织中IL-1α基因的表达[J]. , 1997, 15(01): 0-.

3种铸造合金全冠戴用后Ki67和P53蛋白在牙龈组织中的表达

Expression of Ki67 and P53 proteins in gingival tissue after wearing casting alloy crowns

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

本文评价