血小板衍生生长因子-BB与胰岛素样生长因子-Ⅰ联合应用对大鼠正畸牙牙周膜细胞整合素β3表达的影响

doi:10.3969/j.issn.1000-1182.2011.04.019

华西口腔医学杂志

血小板衍生生长因子-BB与胰岛素样生长因子-Ⅰ联合应用对大鼠正畸牙牙周膜细胞整合素β3表达的影响

苏牧1，2 刘建国1 管晓燕1 黄瑾1 徐宇红1 张疆弢1 彭菊香1 李高华1 钟建莉1

1.遵义医学院口腔医院正畸科，遵义563003； 2.航空总医院口腔科，北京100012

收稿日期:2011-08-25 修回日期:2011-08-25 出版日期:2011-08-20 发布日期:2011-08-20
通讯作者: 刘建国，Tel：0852-8609806
作者简介:苏牧（1973—），女，湖南人，主治医师，硕士
基金资助:
贵州省自然科学基金资助项目[黔科合J字（2009）2177号]；贵州省卫生厅基金资助项目（gzwkj2008-030号）

Combined effects of platelet-derived growth factor-BB and insulin-like growth factor-I on the expression of integrin β3 protein in periodontal ligament cells of rats during orthodontic tooth movement

Su Mu1，2, Liu Jianguo1, Guan Xiaoyan1, Huang Jin1, Xu Yuhong1, Zhang Jiangtao1, Peng Juxiang1, Li Gaohua1, Zhong Jianli1

1. Dept. of Orthodontics, School of Stomatology, Zunyi Medical College, Zunyi 563003, China; 2. Dept. of Stomatology, Aviation General Hospital, Beijing 100012, China）

Received:2011-08-25 Revised:2011-08-25 Online:2011-08-20 Published:2011-08-20
Contact: Liu Jianguo，Tel：0852-8609806

摘要/Abstract

摘要：

目的观察血小板衍生生长因子-BB（PDGF-BB）与胰岛素样生长因子-Ⅰ（IGF-Ⅰ）联合应用对大鼠正畸牙压力侧牙周膜细胞（PDLCs）中整合素β3蛋白表达的影响。方法建立SD大鼠正畸牙移动模型，隔日于正畸牙颊侧牙龈黏膜下单独或联合注射10 ng PDGF-BB及200 ng IGF-Ⅰ，加力10 d后处死大鼠，取材用免疫组织化学方法检测正畸牙压力侧牙周膜细胞中整合素β3的表达。结果PDGF-BB、IGF-Ⅰ单独或联合应用均能增强压力侧牙周膜细胞中整合素β3表达（P<0.01）；与PDGF-BB、IGF-Ⅰ单独应用比较，IGF-Ⅰ加PDGF-BB组牙周膜细胞中整合素β3表达明显增强（P<0.05和P<0.01）。结论外源性PDGF-BB和IGF-Ⅰ在大鼠正畸牙的局部注射能上调压力侧牙周膜细胞中整合素β3表达，二者联合应用具有协同效应。

关键词: 血小板衍生生长因子-BB, 胰岛素样生长因子-Ⅰ, 整合素β3

Abstract:

Objective To investigate the combined effects of platelet-derived growth factor-BB（PDGF-BB） and insulin-like growth factor-Ⅰ（IGF-Ⅰ） on the expression of integrin β3 protein in the periodontal tissues of the compressing side of orthodontic tooth in rats. Methods Establishing orthodontic movement model in Sprague-Dawley rats， which were injected with 10 ng PDGF-BB，200 ng IGF-Ⅰ alone or in combination in the buccal submucosal area of the orthodontic tooth. The injection was applied every other day. The experiment continued for ten days and then the rats were sacrificed. The expression of integrin β3 protein in periodontal ligament tissues of the compressing side was detected by immunohistochemical techniques. Results The expression of integrin β3 protein in periodontal ligament tissues of the compressing side of each experimental group was significantly increased compared with that of the control group（P<0.01）. Meanwhile the maximum mean optical density value of integrin β3 protein expression was attained in the combination group which showed a significant increase compared with the PDGF-BB group（P<0.05） and the IGF-Ⅰ group（P<0.01）. Conclusion The combination of exogenous PDGF-BB and IGF-Ⅰ in orthodontic tooth movement may enhance the expression of integrin β3 protein in periodontal ligament cells and PDGF-BB and IGF-Ⅰmay have a synergistic effect during orthodontic tooth movement.

Key words: platelet-derived growth factor-BB, insulin-like growth factor-Ⅰ, integrin β3

苏牧刘建国管晓燕黄瑾徐宇红张疆弢彭菊香李高华钟建莉. 血小板衍生生长因子-BB与胰岛素样生长因子-Ⅰ联合应用对大鼠正畸牙牙周膜细胞整合素β3表达的影响[J]. 华西口腔医学杂志, doi: 10.3969/j.issn.1000-1182.2011.04.019.

Su Mu1，2, Liu Jianguo1, Guan Xiaoyan1, Huang Jin1, Xu Yuhong1, Zhang Jiangtao1, Peng Juxiang1, Li Gaohua1, Zhong Jianli1. Combined effects of platelet-derived growth factor-BB and insulin-like growth factor-I on the expression of integrin β3 protein in periodontal ligament cells of rats during orthodontic tooth movement[J]. West China Journal of Stomatology, doi: 10.3969/j.issn.1000-1182.2011.04.019.

[1]	黄瑾刘建国宋琦苏牧张疆弢管晓燕彭菊香. 血小板衍生生长因子-BB、转化生长因子-β1联合应用对大鼠正畸牙牙周膜中整合素β3表达的影响[J]. 华西口腔医学杂志, 2014, 32(4): 413-417.
[2]	李彦牛忠英汤楚华包博师天鹏司少艳. 三维培养模型中胰岛素样生长因子-Ⅰ对人牙周膜细胞增殖及碱性磷酸酶活性的影响[J]. 华西口腔医学杂志, 2011, 29(03): 229-232.
[3]	张京剧;陈扬熙;肖立伟;段培佳;赵青. 大鼠牙周炎牙正畸移动后整合素β_3mRNA变化的实验研究[J]. 华西口腔医学杂志, 2006, 24(02): 106-109.
[4]	韩为农,彭解英,徐锡萍,彭隆祥,刘蜀凡. 血小板衍生生长因子BB及其受体在口腔粘膜下纤维化的超微结构定位[J]. 华西口腔医学杂志, 2002, 20(04): 254-256.

血小板衍生生长因子-BB与胰岛素样生长因子-Ⅰ联合应用对大鼠正畸牙牙周膜细胞整合素β3表达的影响

Combined effects of platelet-derived growth factor-BB and insulin-like growth factor-I on the expression of integrin β3 protein in periodontal ligament cells of rats during orthodontic tooth movement

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