基质金属蛋白酶-3和金属蛋白酶组织抑制因子-1在兔下颌牵张成骨改建期的表达及意义

华西口腔医学杂志

基质金属蛋白酶-3和金属蛋白酶组织抑制因子-1在兔下颌牵张成骨改建期的表达及意义

邹淑娟,王志国,胡静,李继华

610041四川大学华西口腔医院口腔正畸科(邹淑娟), 四川大学华西口腔医院口腔颌面外科(王志国,胡　静,李继华)

收稿日期:2003-10-25 修回日期:2003-10-25 出版日期:2003-10-20 发布日期:2003-10-20
基金资助:
本课题为高等学校博士点专项科研基金(编号20020610062)及四川大学校基金(2002年)资助项目

Expression of Matrix Metalloproteinase-3 and Tissue Inhibitor Metalloproteinases-1 in Regenerated Rabbit Bone after Mandibular Osteodistraction

ZOUShujuan*,WANG Zhiguo,HUJing,et al

*Department ofOrthodontics,West China College ofStomatology,Sichuan University,Chengdu610041,China

Received:2003-10-25 Revised:2003-10-25 Online:2003-10-20 Published:2003-10-20

摘要/Abstract

摘要：

目的　研究基质金属蛋白酶-3(MMP-3)和金属蛋白酶组织抑制因子-1(TIMP-1)在下颌骨牵张后形成的新骨组织中的定位表达。方法　对8只成熟大耳白兔按每天1 mm的牵张速率延长双侧下颌骨6 mm,在牵张结束后第4周处死动物,取牵张区新骨标本作组织学和免疫组化检测。结果　MMP-3与TIMP-1在牵张骨痂中均呈高水平表达,MMP-3阳性信号主要定位于新生骨小梁边缘的成骨细胞和埋入新骨基质中的骨细胞,TIMP-1则定位于成骨细胞。结论　MMP-3及TIMP-1可协同调节胞外基质的降解来影响牵张成骨的改建过程。

关键词: 下颌骨牵张, 基质金属蛋白酶-3, 金属蛋白酶组织抑制因子-1, 骨改建

Abstract:

Objective　To observe the expression patterns of matrix metalloproteinase-3 and its inhibitor, tissue inhibitor metalloproteinases (TIMP)-1, in remodeling phase of mandibular distraction osteogenesis.Methods　Bilateral mandibular osteot- omies were performed in 8 mature rabbits. The mandibles were lengthened 6 mm using a custom-made distractor with a rate of 1 mm/d. All animals were killed in 4 weeks after completion of distraction. The distracted calluses were harvested and processed for histology and immunohistochemistry of MMP-3 and TIMP-1.Results　Elevated expression of both MMP-3 and TIMP-1 was found in the distracted calluses resulting frommandibular lengthening. Positive staining forMMP-3 was seen in the osteoblasts and osteocytes, and TIMP-1 was mainly localized in osteoblasts.Conclusion　MMP-3 and TIMP-1 appear to play important roles in the remodeling of new bone created by mandibular distraction osteogenesis.

Key words: mandibular osteodistraction, matrixmetalloproteinase-3, tissue inhibitormetalloproteinases-1, bone re- modeling

邹淑娟,王志国,胡静,李继华. 基质金属蛋白酶-3和金属蛋白酶组织抑制因子-1在兔下颌牵张成骨改建期的表达及意义[J]. 华西口腔医学杂志.

ZOUShujuan*,WANG Zhiguo,HUJing,et al. Expression of Matrix Metalloproteinase-3 and Tissue Inhibitor Metalloproteinases-1 in Regenerated Rabbit Bone after Mandibular Osteodistraction[J]. West China Journal of Stomatology.

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