关键词: 基因重组人骨形成蛋白-2, 牛骨形成蛋白, 珊瑚/聚乳酸, 复合人工骨, 成骨活性

Abstract:

Objective　To study the effects of artificial bone composite of bicoral,rhBMP-2 and PLA in repairing calvarial critical-size defects.Methods　Calvarial defects in 24 rabbitswere surgically made and then half of the defectswere repaired with the artificial composite bone. Another half of themwere repairedwith bicoral/PLA composite and served as controls. Four rabbits in each group were sacrificed at 4,8,12 weeks after operation, respectively. The treatment effects were evaluated with scanning electron microscopy and mechanical strength testing.Results　New bone was observed not only in the periphery, but also inside the artificial bone in both groups, but earlier and more newbone formationwas observed in treatment group compared with control group. The mechanical strength test showed that the artificial bone in two groups, which had same mechanical strength before im- plantation, had significant different mechanical strength after operation. The strength of the artificial composite bone was higher than that of controls and was same with normal rabbit calvarial bone.Conclusion　The artificial composite bone possess a highly repairing ability, and the healing in bone defects may be accomplished by both osteoinductive and osteoconductive mechanism. The material may be used as a good substitute for bone grafting.

Key words: recombinant human bone morphogenetic protein-2, bovine bone morphogenetic protein, coral/polyactic acid, osteoconductive