关键词: 细胞培养, 人牙周膜成纤维细胞, 四环素

Abstract:

Objective To explore the biological effects of tetracycline on cultured human periodontal ligament fibroblasts (HP- DLFs ).Methods Increasing concentrations of tetracycline(1,5,20,100,500,2 500卿ml) were added to the medium of cul- tured HPDLFs, respectively. After co-incubated for 2 days, cell morphology~observed under reverse microscope, meanwhile, cell proliferation activity~assayed using M TI', the total amount of protein~detected with Coumassie Bright Blue method and DNA synthesis~measured场3H-TdR. Results Over a concentration range of 1 to 100留ml, cells demonstrated a normal appearance, spindle or fusiform shaped. Moreover, at a concentration range of 20 to 100 pg/ml, tetracycline significantly en- hanced the proliferating act州ty and biosynthesis‘1-II〕I〕 Us(P<0.01).However, higher concentration (2 500 tcg/ml) not only changed cell morphology, but also significantly inhibited cellular activity. Conclusion The results suggested that proper doses of tetracycline could promote proliferation and biosynthesis of HPDLFs while higher concentrations of tetracycline had cytotoxic effect