关键词: 人白细胞介素-1受体拮抗剂, 基因转染, 阳离子脂质体

Abstract:

Objective  To investigate the expression of the human interleukin-1 receptor antagonist（hIL-1ra） in the transfected chondrocytes of temporomandibular joint（TMJ）. Methods  Chondrocytes of TMJ in vitro were transfected by hIL-1ra gene via cationic liposome as a medium. The stable transfected cells were selected by G418. The proliferations of the transduced cell were examined with the growth curve, cell population doubling time. The protein expressing in different periods was detected by immunocytochemistry and enzyme-linked immunosorbent assay（ELISA）. Results  The proliferation suppression of gene transfected cells fell significantly with compared to normal cells. The expression of hIL-1ra was detected in the cell plasma and the cell culture supernatant. The highest expression of IL-1ra protein was at the time of 48 hours after gene transfection. The transiently transfected cells were secreted IL-1ra protein continuously 28 days and the stably transduced cells were secreted IL-1ra protein till 72 days. Conclusion  This study showed that hIL-1ra protein expressed positively in the cell plasma and the culture supernatant after gene transfection within a certain periods.

Key words: human interleukin-1 receptor antagonist, gene transfection, cationic liposome