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Table of Content
01 June 2022, Volume 40 Issue 3
  • Experts’ consensus on precaution and treatment for complications of sagittal split ramus osteotomy
    Zhu Songsong, Wang Xudong, Yang Xuewen, Wang Xiaoxia, Tian Lei, Liu Shuguang, Zheng Guangsen, Tang Zhenglong, Wu Guomin, Li Zhiyong, Bai Xiaofeng, Huang Xuanping, Huang Li, Xi Weihong, Zhu Yaomin
    West China Journal of Stomatology. 2022, 40(3):  247-254.  doi:10.7518/hxkq.2022.03.001
    Abstract ( 988 )   HTML ( 416 )   PDF(pc) (879KB) ( 1021 )   Save
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    Sagittal split ramus osteotomy (SSRO) is a versatile orthognathic procedure for correcting mandibular deformities. Various complications can possibly occur when performing SSRO, and it can even cause serious adverse consequences because of the complexity of anatomy and operative procedures. The types of complications and their accompanying clinical manifestations are closely related to the choice of diagnosis and treatment strategies and clinical outcomes. To discuss the causes, prevention, and treatment measures of various common complications of SSRO, domestic orthognathic surgery experts prepared this consensus to increase the awareness of SSRO complications, thereby ensuring safe surgical procedure and good results.

    Experts’ consensus on perioperative management of tooth extractions in patients receiving oral antithrombotic treatment
    Pan Jian, Xue Yang, Zhao Jihong, Zhou Qing, Zou Duohong, Chen Songling, Han Bing, Cui Nianhui, Liu Xian, Wang Liao, Hu Kaijin
    West China Journal of Stomatology. 2022, 40(3):  255-263.  doi:10.7518/hxkq.2022.03.002
    Abstract ( 3051 )   HTML ( 565 )   PDF(pc) (967KB) ( 3688 )   Save
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    Thromboembolic diseases, which comprise venous thromboembolic diseases and arterial thromboembolic diseases, have become the number one cause of death worldwide. To prevent or treat thrombosis, patients with thromboembolic diseases need to take antithrombotic drugs, which would increase the risk of bleeding during and after surgery. Tooth extraction is the most common operation in oral and maxillofacial surgery clinics. Although patients given oral antithrombotic drugs do not need to undergo drug withdrawal, the perioperative management of such patients remains confusing to most clinicians. Moreover, the potential risk factors for bleeding warrant further study. To improve the clinicians’ knowledge of perioperative management for patients subjected to tooth extractions with oral antithrombotic drugs, experts have drafted this consensus focusing on preoperative bleeding risk assessment, intraoperative operating norms, and postoperative care to summarize the points needing attention.

    Experts’ consensus on space management of mixed dentition
    Guo Weihua, Wang Jun, Chen Xu, Wang Xiaojing, Zhao Wei, Song Guangtai, Wu Li’an, Jiang Beizhan, Zhang Qiong, Wang Jun, Li Yu, Zhao Ning, Tan Jiali, Li Huang, Shu Rui, Zhou Chenchen, Fu Lei, Chen Xuepeng, Zou Jing
    West China Journal of Stomatology. 2022, 40(3):  264-270.  doi:10.7518/hxkq.2022.03.003
    Abstract ( 2096 )   HTML ( 398 )   PDF(pc) (915KB) ( 2739 )   Save
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    The mixed dentition stage is the period between primary and permanent dentition. The following biological processes are complicated and variable: jaw growth, development of inherited permanent teeth embryo, physiological absorption of primary teeth, restoration of surrounding alveolar bones, and growth and function establishment of soft tissues. For the normal development of the jaw, the establishment of the good occlusion relationship, development, and function of soft tissue is very important, whether or not the primary teeth are normally replaced by the permanent teeth in the mixed dentition stage. The eruption space is linked to the normal replacement of primary and permanent teeth. The presence of a mixed dentition space results in the incidence and progression of malocclusion and impacts the normal growth and development of the occlusion, jaw, and face. Space management in the mixed dentition stage is a crucial means to prevent and reduce malocclusion. The following were discussed and analyzed: the possible space problems, why the size of the space was affected, the content that needs to be assessed, and the methods of space management in the mixed dentition that can be used to unify and standardize the management of mixed dentition. This paper was developed to serve as a guide for regulated space management during the mixed dentition period.

    Vessel anastomosis in free flap reconstruction for oral and maxillofacial defects: techniques and key points
    Li Chunjie, Han Bo, Zhu Guiquan.
    West China Journal of Stomatology. 2022, 40(3):  271-278.  doi:10.7518/hxkq.2022.03.004
    Abstract ( 1628 )   HTML ( 283 )   PDF(pc) (3724KB) ( 1657 )   Save
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    The quality of vessel anastomosis is a key element for the success of free flap reconstruction. When adopting free flaps for oral and maxillofacial defect reconstruction, skills in selecting vessels, determining the clinical manifestations of the recipient vessel, and anastomosis technique are needed. Key points on postoperative flap observation were also required. We are experienced on this subject given that we have accomplished more than 1 000 free flaps for patients with oral and maxillofacial defects in the last 5 years. In this article, we summarize the skills and key points in free flap reconstruction of oral and maxillofacial defects, including vessel anastomosis skills, vessel selection, and vessel crisis diagnosis.

    Association of soluble epoxide hydrolase 2 gene with the risk of non-syndromic cleft lip with or without cleft palate in western Han Chinese population
    Yang Mengxi, Wang Yiru, Yin Bin, Zheng Qian, Shi Bing, Jia Zhonglin.
    West China Journal of Stomatology. 2022, 40(3):  279-284.  doi:10.7518/hxkq.2022.03.005
    Abstract ( 189 )   HTML ( 28 )   PDF(pc) (1295KB) ( 43 )   Save
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    Objective

    This study aimed to explore the associations between soluble epoxide hydrolase 2 gene (EPHX2) variants and non-syndromic cleft lip with or without cleft palate (NSCL/P) in Chinese Han population.

    Methods

    We recruited 159 NSCL/P cases from Chinese Han population and carried out targeted resequencing using the whole genome sequencing data of 542 healthy Chinese individuals from Novegene internal database as controls. We classified EPHX2 variants as common or rare according to their minor allele frequency and performed an association analysis for common variations and a burden analysis for rare variations.

    Results

    The lowest P-value in NSCL/P was observed at rs57699806 (P=0.000 13, OR=2.849 and 95% CI: 1.691-4.800), followed by rs4732723 (P=0.006 50, OR=0.662 and 95%CI: 0.491-0.892), rs7829267 (P=0.009 20, OR=1.496 and 95%CI: 1.117-2.005), rs721619 (P=0.011 00, OR=1.474 and 95%CI: 1.098-1.980), and rs7816586 (P=0.040 00, OR=1.310 and 95%CI: 1.015-1.691). The odds ratios suggested the C allele at rs4732723 as a protective factor for NSCL/P and the reference alleles at other single nucleotide polymorphisms (SNPs) as the risk factors for NSCL/P. Burden analysis showed no statistical significance (P>0.05).

    Conclusion

    Through targeted resequencing, this study identified five SNPs named rs57699806, rs4732723, rs7829267, rs721619, and rs7816586 around the region of EPHX2 gene associated with NSCL/P in Chinese Han population. Four SNPs of rs57699806, rs4732723, rs7829267, and rs7816586 were first identified.

    Promoting the adhesion of human gingival epithelial cells on titanium surface by non-thermal atmospheric plasma irradiation
    Zhang Min, Ao Xiaogang, Zheng Zheng, Chen Wenchuan.
    West China Journal of Stomatology. 2022, 40(3):  285-292.  doi:10.7518/hxkq.2022.03.006
    Abstract ( 165 )   HTML ( 30 )   PDF(pc) (2666KB) ( 70 )   Save
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    Objective

    This work aimed to study the biological behavior of human gingival epithelial cells (HGECs) irradiated by non-thermal atmospheric plasma (NTAP) on a titanium surface.

    Methods

    Cultured HGECs (3⁃5 generations) with the best activity were digested and treated for varying times (0, 10, 20, 30, and 60 s) by NTAP and then seeded on the surface of a titanium disc. The HGECs were cultured in oral keratinocyte medium and 1% penicillin/streptomycin solution. The cells were kept in an atmosphere of 5% CO2 at 37 ℃ and incubated for different times (4, 12, 24, and 48 h; n=5). Cell counting kit-8 (CCK-8) was used to detect cell adhesion capacity. Scanning electron microscopy (SEM) was conducted to observe the morphology of cells on titanium plates. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were used to evaluate the gene expression of adhesion-related molecules, such as Laminin α3, Integrin β4, and Plectin.

    Results

    The number of adhered cells increased at 0‑20 s, whereas that gradually decreased at 20⁃60 s. Therefore, cell culture at the two time points showed that HGECs adhesion reached the maximum when NATP was irradiated for 20 s. Compared with the control group, more cells in the treatment group adhered to the titanium surface at each time point (P<0.05). Cells in the treatment group showed more irregular polygons, more protrusions and pseudopods, and a larger cell diffusion area on the titanium surface than those in the control group. qRT-PCR showed that the expression levels of Laminin α3, Integrin β4, and Plectin adhesion-related genes on the titanium surface in the treatment group were higher than those in the control group at each culture time point (P<0.05). Western blot showed that the expression levels of Laminin α3, Integrin β4, and Plectin adhesion-related proteins on the titanium surface were higher in the treatment group than in the control group at 4 and 12 h.

    Conclusion

    After NTAP treatment, the results showed that 20 s of treatment time could maximize the number of adhered cells on the titanium surface; change the cell adhesion morphology; and significantly upregulate the expression of adhesion-related genes and proteins of Laminin α3, Integrin β4, and Plectin. Furthermore, it could promote the biological sealing effect of HGECs on the titanium surface.

    Comparison of interleukin expression in gingival crevicular fluid between patients with invisible orthodontics treat-ment and fixed orthodontics treatment
    Luo Houzhuo, Fang Shishu, Liu Qian, Dang Wei, Wang Yanli
    West China Journal of Stomatology. 2022, 40(3):  293-296.  doi:10.7518/hxkq.2022.03.007
    Abstract ( 222 )   HTML ( 40 )   PDF(pc) (636KB) ( 135 )   Save
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    Objective

    To compare the expression levels of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-16, and IL-18 in gingival crevicular fluid between patients with invisible orthodontics treatment and fixed orthodontics treatment.

    Methods

    A total of 67 patients with invisible orthodontic treatment were selected as the observation group, and 40 patients with fixed orthodontic treatment were selected as the control group. The expression levels of IL-1β, IL-6, IL-8, IL-10, IL-16, and IL-18 in gingival crevicular fluid before, 24 h, and 12 months after orthodontic treatment were detected.

    Results

    No significant difference in basic characteristics and interleukin expression levels in gingival crevicular fluid was observed between the two groups before orthodontic treatment (P>0.05). After 24 h of orthodontic treatment, the expression levels of IL-1β, IL-6, IL-8, IL-10, and IL-18 in gingival crevicular fluid increased in both groups; however, no significant difference was observed between the two groups (P>0.05). After 12 months of orthodontic treatment, the expression levels of IL-1β, IL-6, IL-8, IL-10, and IL-18 in gingival crevicular fluid in the observation group were significantly lower than those in the control group (P<0.05), and no significant difference in the expression level of IL-16 was observed between the two groups (P>0.05).

    Conclusion

    Compared with patients with fixed orthodontics treatment, those with invisible orthodontics treatment had weaker oral inflammatory response, which was conducive to the recovery of the oral microenvironment.

    Comparing accuracy after guide access and microscope-assisted access for fiber post removal
    Cai Pingping, Chen Xi, Jiang Yi, Lu Zhaojie, Lin Jie, Zheng Zhiqiang.
    West China Journal of Stomatology. 2022, 40(3):  297-302.  doi:10.7518/hxkq.2022.03.008
    Abstract ( 431 )   HTML ( 68 )   PDF(pc) (2294KB) ( 320 )   Save
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    Objective

    This study compared the accuracy of the guide-supported and the microscope-assisted fiber post removal systems by using the extracted teeth. These new idea and theory can be used by clinicians to remove fiber posts.

    Methods

    Twenty-eight human extracted premolars were randomly divided into the guide and microscope groups. After root canal treatment and fiber post restoration, the fiber posts were removed by using a digital guide and via microscope-assisted ultrasonic instrument, respectively. Mimics 10.0 was used to measure the deviation, and the accuracy of the two fiber post removal systems were compared.

    Results

    In the guide group, the apical vertical deviation was 0.99 mm±0.52 mm, the apical horizontal deviation was 0.75 mm±0.19 mm, the angle deviation was 2.32°±0.64°, and the volume loss was 8.09 mm3±1.42 mm3. In the microscope group, the apical vertical deviation was 0.44 mm±0.23 mm, the apical horizontal deviation was 0.23 mm±0.07 mm, the angle deviation was 0.64°±0.31°, and the volume loss was 15.25 mm3±3.94 mm3. No significant difference was found in the apical vertical deviation between the two groups (P>0.05), whereas the apical horizontal deviation, the angle deviation, and the volume loss were significantly different between the two groups (P<0.05).

    Conclusion

    The removal of fiber post supported by a digital guide helped reduce the volume loss of post-core restoration teeth, but its accuracy was lower than that of removal by using a microscope-assisted ultrasonic instrument.

    Preliminary efficacy analysis of block iliac bone grafting in patients with alveolar cleft in mixed dentition
    Jiang Xiaoxian, Mao Chuanqing, Lai Yongzhen, Lu Meng, Wang Chengyong, Cai Zhiyu, Chen Weihui.
    West China Journal of Stomatology. 2022, 40(3):  303-308.  doi:10.7518/hxkq.2022.03.009
    Abstract ( 192 )   HTML ( 28 )   PDF(pc) (3120KB) ( 100 )   Save
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    Objective

    To investigate the efficacy of block iliac bone grafting in patients with unilateral alveolar cleft in mixed dentition.

    Methods

    A retrospective study was conducted on patients with unilateral alveolar clefts in mixed dentition who were treated in the Department of Oral and Maxillofacial Surgery, Fujian Medical University Union Hospital. All patients underwent unilateral alveolar cleft bone graft repair with autogenous block iliac bone blocks. The healing of bone blocks was analyzed at 1 week and 6-12 months after surgery. Mimics software was used for the three-dimensional reconstruction and volumetric measurement of the iliac bone blocks on the follow-up imaging data of 15 patients aged 9-12 years without the eruption of canines before surgery, and the bone resorption rate of the iliac bone blocks was comparatively analyzed.

    Results

    In the 37 patients, bone grafting was successful in 32 and failed in five. The success rate of bone grafting was 86.5%. In 15 patients aged 9-12 years without the eruption of canines before surgery, eruption through the bone graft area was observed in two patients 6-12 months after the operation. Cone beam computer tomography showed that the grafted bone block exhibited good bony connections, and its resorption mainly occurred on the crests and palatal sides of the alveolar ridge. Bone resorption rates varied considerably between patients with a mean bone resorption rate of 39.0%±13.8% at 6-12 months after surgery.

    Conclusion

    For patients in mixed dentition, bone grafting with block iliac bone can achieve better osteogenesis effect.

    Comparison of surgical effects between the modified West China method and Millard method in repairing unila-teral cleft lip
    Liu Xiaolin, Wang Yu, Feng Xiaowei, Liu Wenjing, Li Xiangjun.
    West China Journal of Stomatology. 2022, 40(3):  309-313.  doi:10.7518/hxkq.2022.03.010
    Abstract ( 526 )   HTML ( 85 )   PDF(pc) (1665KB) ( 419 )   Save
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    Objective

    The purpose of this study is to compare the surgical effects of the modified West China method and Millard methods for repairing unilateral cleft lip.

    Methods

    A total of 34 cases of unilateral cleft lip were analyzed, composed of 16 cases in the experimental group subjected to the modified West China method and 18 cases in the control group, which was subjected to the Millard method. Photographs before and after operation were compared, and SPSS 21.0 was used in statistical analysis.

    Results

    After operation, no significant difference in symmetry between the healthy and affected sides of the nasal alar foot (sba), nasal alar point (al), lip peak point (cph), and mouth angle point (ch) in the experimental group (P>0.05), but no significant difference in symmetry was found between healthy and affected sides of cph and ch in the control group (P>0.05). No significant differences in the symmetry rates of the sba, cph, ch and the nasal columella regression rates were found between the experimental and control groups (P>0.05). The symmetry rates of the al in the experimental group were higher (P<0.05).

    Conclusion

    For unilateral cleft lip repair, two methods had better effects on lip repair, but the nasal symmetry showed greater improvement when the modified West China me-thod was used than when the Millard method was used.

    Evaluation of changes in orbital volume in adult female patients with maxillary transverse deficiency treated with a maxillary skeletal expander
    Shi Xiaoyang, Lin Xuefen, Ma Chi, Chen Muhan, Liu Dongxu.
    West China Journal of Stomatology. 2022, 40(3):  314-319.  doi:10.7518/hxkq.2022.03.011
    Abstract ( 649 )   HTML ( 78 )   PDF(pc) (1801KB) ( 408 )   Save
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    Objective

    The effects of using a maxillary skeletal expander (MSE) on the orbital volume and width between periorbital bones in the treatment of adult female patients with maxillary transverse deficiency (MTD) were evalua⁃ted.

    Methods

    A total of 20 adult female patients with MTD with an average age of (22.60±6.29) years were included in the study. The patients were treated with MSE. Cone beam computed tomography was performed before expansion (T0) and no more than 3 weeks after expansion (T1). Orbital volume and periorbital bone width were measured with Mimics 21.0 and analyzed with SPSS 20.0. Paired t-test was performed, and a P value of <0.05 indicated significant difference.

    Results

    After expansion, the orbital volume increased by (346.80±275.31) mm3 (P<0.05). The width between the right and left zygomaticomaxillary sutures increased by (1.69±0.57) mm (P<0.05), and the width between the right and left infraorbital points increased by (1.71±0.70) mm (P<0.05). However, the width between the right and left frontozygomatic sutures increased by (0.15±0.32) mm (P>0.05). Finally, the width between the right and left supraorbital points increased by (0.23±0.52) mm (P>0.05).

    Conclusion

    The maxillary skeletal expander slightly expanded the orbital volume in the adult female patients and increased the lateral widths of the periorbital bones.

    Effect of protein kinase D inhibitor CRT0066101 on the cell migration of salivary adenoid cystic carcinoma
    Chen Jiao, Die Lü, Chen Hongli, Zhang Ping.
    West China Journal of Stomatology. 2022, 40(3):  320-327.  doi:10.7518/hxkq.2022.03.012
    Abstract ( 136 )   HTML ( 38 )   PDF(pc) (3951KB) ( 32 )   Save
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    Objective

    This study aimed to study the effect of the protein kinase D (PKD) inhibitor CRT0066101 on the cell migration of salivary adenoid cystic carcinoma (SACC) cells in vitro and explore its related mechanisms to provide new strategies into the clinical treatment of SACC cells.

    Methods

    SACC-LM cells were treated with different concentrations of CRT0066101, and the effect of active phospho-PKD was detected through Western blot and cell immunofluorescence staining. Transwell assay was performed to test cell migration. The effect of CRT0066101 on the protein expression related to the epithelial mesenchymal transition (EMT) was detected through Western blot, cell immunofluorescence staining, and quantitative real-time polymerase chain reaction (qRT-PCR). The cells were treated with the proteasome inhibitor after CRT0066101 administration, and the expression of Snail protein was detected by Western blot.

    Results

    CRT0066101 inhibited PKD activity and reduced the number of invaded cells in SACC-LM cells. CRT0066101 decreased the expression of N-cadherin and Snail and increased the expression of E-cadherin in SACC-LM cells. The regulation of snail protein degradation by CRT0066101 was dependent on the proteasome pathway.

    Conclusion

    CRT0066101 can inhibit the migration of SACC-LM cells in SACC and regulate the expression of proteins and genes related to EMT. The mechanism may be associated with the proteasome-dependent degradation of Snail.

    Systematic review of risk factors of postoperative dysphagia in patients with oral cancer
    Lu Qian, Guo Liumei, Bi Xiaoqin
    West China Journal of Stomatology. 2022, 40(3):  328-334.  doi:10.7518/hxkq.2022.03.013
    Abstract ( 188 )   HTML ( 39 )   PDF(pc) (1998KB) ( 68 )   Save
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    Objective

    This study aims to identify risk factors of postoperative dysphagia in patients with oral cancer by systematic review.

    Methods

    Cohort studies in Chinese or English on risk factors of postoperative dysphagia in patients with oral cancer were searched from CNKI, Wanfang database, VIP Chinese Journal Database, China Biomedical Literature Service System, Chinese Clinical Trial Registry, PubMed, Cochrane Library, EMBASE, Web of Science, and Clinical Trials from the beginning to June 30, 2021. Subject words combined with free words were used to retrieve related articles. The included studies were evaluated, and the effective data were processed with Revman 5.3.

    Results

    Ten studies were selected, and they included 1 241 patients consisting of 473 patients in the exposed group and 768 patients in the control group. After the meta-analysis, the risk factors with statistical significance were as follows: age>60 years, tumor located in oropharynx or mouth floor, tumor size of T3 or T4, TNM stage of Ⅳ, resection involving suprahyoid muscle or tongue resection>50%, combination of neck dissection, tracheotomy, or reconstruction, and postoperative radiotherapy.

    Conclusion

    Age>60 years, tumor in oropharynx or mouth floor, tumor size of T3 or T4, TNM stage of Ⅳ, resection involving suprahyoid muscle or tongue resection>50%, combination of neck dissection, tracheotomy, or reconstruction, and postoperative radiotherapy were significant risk factors of postoperative dysphagia in patients with oral cancer.

    The prognostic value of preoperative peripheral blood inflammatory indicators for squamous cell carcinoma of tongue
    Qin Shuo, Li Chunmei, Li Ran, Hu Shuang, Li Guanghui, Sun Minglei
    West China Journal of Stomatology. 2022, 40(3):  335-340.  doi:10.7518/hxkq.2022.03.014
    Abstract ( 158 )   HTML ( 29 )   PDF(pc) (1141KB) ( 46 )   Save
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    Objective

    A study was conducted to investigate the value of preoperative peripheral blood inflammatory indicators in the prediction of tongue squamous cell carcinoma (TSCC) prognosis.

    Methods

    This retrospective analysis included 210 patients who underwent radical resection for TSCC in the Department of Oral and Maxillofacial Surgery of The First Affiliated Hospital of Zhengzhou University from January 2010 to December 2017. Receiver operating characteristic curve was conducted to determine the best cut-off values of platelet/lymphocyte ratio (PLR) and neutrophil/lymphocyte ratio (NLR). The Kaplan-Meier method and Log-rank test were conducted for univariate analysis, and the Cox proportional hazard regression model was conducted for multivariate analysis. A Nomogram model was established based on the independent risk factors, which were screened by Cox regression model.

    Results

    The univariate analysis showed that PLR, NLR, tumor differentiation, and T, N, and TNM stages were TSCC’s prognostic factors (P<0.05). Multivariate analysis showed that PLR and N and TNM stages were TSCC's independent risk factors (P<0.05). The C-index of the Nomogram was 0.701 (95%CI: 0.651-0.752). The calibration curve shows that the predicted survival rate of the nomogram was in good agreement with the relative survival rate.

    Conclusion

    Preoperative peripheral blood inflammatory indicators can potentially be used to predict TSCC prognosis.

    Effect of SOX2 gene on the biological characteristics of salivary adenoid cystic carcinoma cells
    Wei Zhuo, Zhou Hao, Fei Wei, Nie Xiong, Li Mingfang
    West China Journal of Stomatology. 2022, 40(3):  341-349.  doi:10.7518/hxkq.2022.03.015
    Abstract ( 139 )   HTML ( 41 )   PDF(pc) (6773KB) ( 32 )   Save
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    Objective

    This study aimed to investigate the effect of transfecting SOX2-shRNA vector lentivirus to SACC cell lines on the biological behavior of salivary adenoid cystic carcinoma (SACC)-LM and SACC-83.

    Methods

    Three types of SOX2-shRNA lentiviral vectors (817, 818, and 819) were constructed and transfected successfully. The shRNA with the best inhibitory effect was screened out and transfected into SACC cells. Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and Western blot were used to detect the expressions of Survivin, E-cadherin, and N-cadherin of SACC-LM and SACC-83. CCK-8 and flow cytometry were used to detect SACC-LM and SACC-83 proliferation and apoptosis. Cell scratch test and Transwell method were used to detect the migration and invasion capabilities of SACC-LM and SACC-83.

    Results

    SOX2-shRNA-819 had the best interference effect among the three lentiviruses. After transfecting SOX2-shRNA-819 into SACC-LM and SACC-83, the expressions of SOX2, Survivin, and N-cadherin were significantly reduced, and that of E-cadherin was significantly increased (P<0.05). The cell proliferation ability decreased, and the number of apoptotic cells increased (P<0.05). The cell migration and invasion ability decreased (P<0.05).

    Conclusion

    shRNA interference technology reduced SOX2 expression while downregulating Survivin expression. These two expressions may be related. Low SOX2 expression inhibits the proliferation, migration, and invasion of SACC cells and promotes the apoptosis of SACC cells. SOX2 may be involved in the epithelial⁃mesenchymal transition process. This study provided a relevant theoretical basis for targeting SOX2 gene therapy in SACC.

    Primordial odontogenic tumour: a case report
    Tang Yueyang, Wan Zixin, Yao Lihong, Wu Lanyan, He Zhixiu, Tang Yaling.
    West China Journal of Stomatology. 2022, 40(3):  350-354.  doi:10.7518/hxkq.2022.03.016
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    Odontogenic primary tumor is a rare benign odontogenic tumor in the maxillofacial area. A case of odontogenic primary basal tumor is reported. The clinicopathological features and treatment principles are discussed in the literature.

    Nasal-type extranodal natural killer/T-cell lymphoma with oral ulcer as the earliest clinical manifestation
    Mu Xinyue, Liu Shutai, Sun Jijun, Liu Xiaolin, Li Yuanyuan, Wang Wenlong
    West China Journal of Stomatology. 2022, 40(3):  355-359.  doi:10.7518/hxkq.2022.03.017
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    Nasal-type extranodal natural killer/T-cell lymphoma (ENKTL-NT) is a special subtype of non-Hodgkin’s lymphoma derived from natural killer cells or cytotoxic T cells. Oral ulcers as the first symptom makes its diagnosis challenging because of its rarity and lack of understanding. We report a case of ENKTL-NT in this paper. We analyzed the clinicopathological features, differential diagnosis, treatment, prognosis and the causes of misdiagnosis to provide a diagnostic basis for dentists to make better clinical diagnosis and treatment.

    Cleidocranial dysplasia: a case report and gene mutation analysis
    Zhang Peng, He Pinghua, Xu Peiqiong, Liao Lan
    West China Journal of Stomatology. 2022, 40(3):  360-364.  doi:10.7518/hxkq.2022.03.018
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    Cleidocranial dysplasia is a rare autosomal dominant hereditary disease that mainly affects the skeletal and dental development and has an incidence rate of about 1∶1 000 000. In this study, a case of cranio-clavicular dysplasia was reported, and related literature was reviewed. RUNX2 6p21.1 NM_001024630.3 Exon4 c.534dupAp.(Val179fs) was identified to be a new frameshift mutation by gene analysis.

    Digital partition bonding for porcelain veneers guided by sequence 3D printing templates
    He Jinxiu, Gao Jing, Liu Chunxu, Xie Chenyang, Yu Jiayi, Yu Haiyang.
    West China Journal of Stomatology. 2022, 40(3):  365-369.  doi:10.7518/hxkq.2022.03.019
    Abstract ( 1066 )   HTML ( 2030469317 )   PDF(pc) (3331KB) ( 1126 )   Save
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    Obtaining optimal enamel bonding is a common recommendation to ensure the retention, edge closure, and clinical service life of porcelain veneers. Bonding surface is determined after tooth preparation. More or less dentin exposure often occurs due to the uneven thickness of the enamel. Identifying the enamel or dentin on the bonding surface and adopting the “selective etching” technique are useful to obtain the maximal bonding strength. Naked eye recognition in “selective etching” has uncertain results and is experience-based, whereas the proposed technique controls the preparation depth through the first target restoration space template to obtain the accurate enamel and dentin subarea. It is mapped to the second bonding template, depending on the treatment carried out on the enamel and dentin surface, to accurately predict the adhesion of porcelain veneers.