Chondrocyte cocultures with stromal vascular fraction of adipose tissue promote cartilage regeneration in vivo

doi:10.7518/hxkq.2020.03.002

Abstract

Abstract:

Objective This study aimed to compare the cartilage regeneration of the stromal vascular fraction (SVF) cells and adipose-derived mesenchymal stem cells (ASCs) cocultured with chondrocytes seeded on the scaffolds. Methods The cellular morphologies and proliferation capabilities on the scaffolds were evaluated. The scaffolds with the coculture of ASCs/SVF and chondrocytes were implanted into the full thickness cartilage defective rabbit joints for 10 weeks. Results The cells seeded into the scaffolds showed good adhesion and proliferation. Implantation with SVF and chondrocytes revealed desirable in vitro healing outcomes. Conclusion The SVF cells were better than ASCs in terms of the formation of cartilage matrix in a coimplantation model. Without in vitro expansion, the SVF cells are good cell sources for cartilage repair.

Key words: chondrocytes, stromal vascular fraction, cocultures, cartilage regeneration

CLC Number:

Q254

Ba Kai, Ni Duan, Wang Xinbo, Wei Xueqin, Li Na, Zheng Lian. Chondrocyte cocultures with stromal vascular fraction of adipose tissue promote cartilage regeneration in vivo [J]. West China Journal of Stomatology, 2020, 38(3): 240-244.

Figures/Tables 4

References 16

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