Abstract:

Objective To study the effect and mechanism of salidroside on salivary adenoid cystic carcinoma （SACC- 2）cells in vitro. Methods To detect the effect of salidroside on SACC- 2 cells growth with CCK- 8 kit, the growth curve of cells were drawn. To detect the expression of cysteine proteinase（Caspase 3 and Caspase 8） and proliferating cell nuclear antigen（PCNA） in SACC - 2 cells, immunohistochemistry staining was used. Results According the results of CCK- 8 kit detected, salidroside could inhibit the proliferation of SACC- 2 cells, IC50 value was （4.99 ±0.23）μg/mL. Growing curve showed that SACC- 2 cells of salidroside groups decreased with extending cell culture time. Immunohistochemistry staining showed that Caspase 3 and Caspase 8 were both strong positive expression in SACC- 2 cells of salidroside groups, and poor positive expression in SACC- 2 cells of control group, the difference was significant（P<0.01）. The expression of PCNA was reverse（P<0.01）. Conclusion Salidroside could inhibit the proliferation of SACC- 2 cells and induce SACC- 2 cell apoptosis in vitro, which could be a kind of antitumor medicine in the future.

Key words: salidroside, salivary adenoid cystic carcinoma, cysteine proteinase, proliferating cell nuclear antigen