Abstract:

Objective　to studythe effect of hepatocyte growth factor on the proliferation of dental pulp cell.Methods　the 4th generation dental pulp cell cultured in vitro was used as target cell. 1~200μg/L hepatocyte growth factor was added in the test group while the pure cell culture DMEMas control. TheMTTmethod and flowcytometrywere applied to assay the proliferation and cell cycle of dental pulp cell of different groups.Results　1~200μg/L hepatocyte growth factor showed promoting effect to the proliferation of pulp cell since the 5th day (P<0·05). 100μg/Lwas found to be the optimal concentration. Also on the 5th day, 100μg/L hepatocyte growth factor decreased the G1 subcycle and increased the S subcycle of dental pulp cell (P<0·05). While on the 3rd day, it had no effect on the cell cycle.Conclusion　hepatocyte growth factor had positive effect on the proliferation of dental pulp cell, with 100μg/L as the optimal concentration.

Key words: hepatocyte growth factor, dental pulp cell, cell proliferation, cell cycle