West China Journal of Stomatology

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Biological Effects of Nano-hydroxyapatite/Polyamide 66 on the Osteoblast

YELing, SUQin, ZHOUXue-dong   

  1. KeyLab. ofOral Biomedical Engineering ofMinistry ofEducation, Sichuan University, Chengdu610041, China
  • Received:2005-04-25 Revised:2005-04-25 Online:2005-04-20 Published:2005-04-20

Abstract:

Objective To evaluate the biological effects of nano-hydroxyapatite/polyamide 66(nHA-PA66) on the growth and activity of osteoblast.Methods MTTassaywas used to determine the growth of osteoblast, enzymatic measure was used to deter- mine the activity of ALP and quantitative RT-PCR (QRT-PCR) to evaluate the changes of osteoclacin mRNA expression in os- teoblasts treated by DMEM eluate of nHA-PA66.Results Osteoblasts of different test groups demonstrated relative proliferation rate ranging from 98%~106% without dose-dependent effect. The ALP activity and osteocalcin mRNA expressionwere similar in test and control groups (P>0.05).Conclusion nHA-PA66 has no negative effects on the osteoblast and its osteoblast-compati- bility is proved.

Key words: nano-hydroxyapatite/polyamide 66, quantitative reverse transcriptase-PCR, osteoblast