Abstract:

Objective　To evaluate the effects of basic fibroblast growth factor(bFGF) on proliferation of periodontal fibroblast- like cellsin vivo.Methods　AU-shaped osseous defectwas produced on the buccal side of the mesial root. Four posterior teeth were conducted in four quadrants. Each quadrant included 4 groups: control, bFGF, expanded polytetrafluoroethylene(ePTFE) membrane, bFGF+ePTFE. Each time the 4 teeth sites in one quadrantwere operatedweekly and each dog experienced 4 times of operations. Bromodeoxyuridine(BrdU) was injected 1 hour priorto sacrificingthe dogs at 4 weeks afterfirst surgery. Immunohisto- chemical method was applied to count the BrdU-labeled fibroblast-like cells.Results　The number of BrdU-labeled cells reached the maximum at the 2nd week among all groups and then, decreased with time. Both bFGF and bFGF+ePTFE treated group had significantly more BrdU+cells than remained control or ePTFE groups (P<0·05) at 1st, 2nd weeks after surgery.Conclusion　 2 weeks after periodontal surgery is active phase of proliferation of periodontal fibroblasts. bFGF enhances fibroblast proliferation in early periodontal wound healing, and in turn accelerate periodontal regeneration.

Key words: fibroblast growth factor, periodontal regeneration, fibroblasts