Abstract:

Objective:The purpose of this studywas to investigate the mechanism of the inhibited effects of anti-human vascularendothe- lial growth factor (VEGF) monoclonal antibodies E11on the growth of buccal mucosal carcinoma.Methods:E11was hypodermic and celiac injected into BALBPc nuPnumice, whichwere transplantedwith buccal carcinoma. The saline was injected as the nega- tive control. Mice were killed on the 18th day and the pathological alteration of tumor was evaluated.Results:Microscopically, massive necrosis and decreased vascular density was observed in tumor tissues treated with E11. Correspondentwith the aforemen- tioned phenomena, degeneration and apoptosis was also found in the tumor cells. For the first time, degeneration and destruction of the vascular endothelial cells in the tumor tissues treated with E11were observed using microscopy and transmission electron mi- croscopy examination.Conclusion:The study demonstrated that E11inhibited the tumor angiogenesis by blocking the action of VEGF and resulted in significantinhibitory effects on the tumorgrowth. The resultwould offer a therapeutic base to the clinical ap- plication of the anti-human VEGF monoclonal antibodies.

Key words: VEGF, angiogenesis, tumor, monoclonalantibody, morphology