West China Journal of Stomatology ›› 2021, Vol. 39 ›› Issue (5): 540-546.doi: 10.7518/hxkq.2021.05.007

Previous Articles    

Effect of acidic culture conditions on the proliferation, apoptosis, and migration ability of human tongue squamous cell carcinoma cells and its related mechanism

Dai Xiaohua(), Wang Guanhua, Lian Xiaoli, Yan Yan, Wang Yue, Zou Huiru, Liu Hao.()   

  1. Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, Tianjin Stomatological Hospital, School of Medicine, Nankai University, Tianjin 300041, China
  • Received:2020-09-07 Revised:2021-07-03 Online:2021-10-01 Published:2021-10-11
  • Contact: Liu Hao. E-mail:jstonehome@163.com;kqlh2013@163.com
  • Supported by:
    Special Project of Tianjin Clinical Medicine Key Discipline(HWZX001);Tianjin Science and Technology Projects(19ZXDBSY00070);Natural Science Foundation of Tianjin City of China(18JCYBJC27000);Project of Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction(2021KLMS13);Correspondence: Liu Hao, E-mail: kqlh2013@163.com

Abstract: Objective

This study aims to explore the effect of acidic culture conditions on the proliferation, apoptosis, and migration ability of human tongue squamous cell carcinoma SCC15 and CAL27 cells and its potential molecular mechanism.

Methods

After acidic culture for different periods, methyl thiazolyl tetrazolium (MTT) method was adop-ted to detect the cell proliferation of SCC15 and CAL27. Flow cytometry was employed to detect the apoptosis level of SCC15 and CAL27 cells. The migration ability of SCC15 and CAL27 after acidic culture was detected by scratch hea-ling test. Real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) was used to detect the mRNA expression of cyclooxygenase 2 (COX-2) and survivin in SCC15 and CAL27 cells after acidic culture.

Results

After culture for 24 h under acidic microenvironment, SCC15 and CAL27 cells grew rapidly and reached the stationary phase after adjustment for 3 days. The apoptosis levels of SCC15 and CAL27 cells decreased after acidic culture, but the most significant reduction occurred after 6 h of acidic culture. The scratch healing rates of SCC15 and CAL27 cells increased after acidic culture. The results of FQ-PCR showed that the mRNA expression levels of COX-2 and survivin in SCC15 and CAL27 cells increased after acidic culture.

Conclusion

Extracellular acidic microenvironment can inhibit the apoptosis of tongue squamous carcinoma cells, promote their migration, and induce more adaptable and malignant tongue squamous carcinoma cells. The mechanism may be related to COX-2 and survivin and their signal pathways.

Key words: tongue squamous cell carcinoma, acidic extracellular environment, cell migration, cell apoptosis

CLC Number: