West China Journal of Stomatology ›› 2021, Vol. 39 ›› Issue (5): 531-539.doi: 10.7518/hxkq.2021.05.006

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Effect of micro/nanoscaled Ti phosphate/Ti oxide hybrid coating on the osseointegration of Ti implants

Zhang Jie(), Zhu Songsong, Jiang Nan.()   

  1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Orthognathic and Temporomandibular Joint Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2020-12-21 Revised:2021-04-19 Online:2021-10-01 Published:2021-10-11
  • Contact: Jiang Nan. E-mail:ttxsjiezhang@163.com;dent_jn@163.com
  • Supported by:
    The National Natural Science Foundation of China(81901026);Correspondence: Jiang Nan, E-mail: dent_jn@163.com

Abstract: Objective

This study was performed to fabricate a bionic coating with titanium (Ti) phosphate to promote the osseointegration of Ti substrate implants.


Phosphorylated micro/nanocoating was prepared on the surface of pure titanium (i.e., TiP-Ti) by hydrothermal process under special pressure, and the untreated smooth pure titanium (cp-Ti) was selected as the control. To evaluate the characteristics of the coating surface, scanning electron microscopy, X-ray diffraction, atomic force microscopy, and contact-angle measurement were performed. In addition, the effects of TiP-Ti on the proliferation, adhesion, and differentiation of rat bone marrow mesenchymal stem cells (BMSCs) were investigated by using in vitro cytology. Finally, TiP-Ti implants were implanted into the rat tibia, and the effect of TiP-Ti on the osseointegration in the host was evaluated after 12 weeks.


The TiP-Ti surface presented a bionic structure with coexisting nanoscale 3D spatial structure and microscale pores. In vitro experiments showed that the BMSCs had enhanced adhesion, proliferation, and osteogenic differentiation on the TiP-Ti surface. Furthermore, in vivo, TiP-Ti showed considerably stronger osseointegration compared with pure titanium, and the ultimate shear strength and maximum pushing force were significantly improved.


A bionic structure with TiP-Ti micro/nanoscale coating was successfully fabricated, indicating a promising method for modifying the surface of implants.

Key words: Ti phosphate, Ti oxide, osseointegration, micro/nanoscale morphology, hierarchical structure

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