Effects of different irradiators on the establishment of osteoradionecrosis model of rat mandible

doi:10.7518/hxkq.2021.05.005

Abstract

Abstract: Objective

To compare the effects of different irradiators on the establishment of osteoradionecrosis of jaw model (ORNJ) to explore an ideal modeling method.

Methods

A total of 33 adult SD rats were included and randomly divided into three groups according to the radiation equipment, namely, the blank control (CN, 3 rats), group A (linear accelerator irradiation, 15 rats), and group B (small-animal irradiator irradiation, 15 rats). Groups A and B were irradiated with daily fractions of 7, 8, and 9 Gy for 5 days and further divided into three subgroups as follows: group A₃₅/B₃₅, 35 Gy; group A₄₀/B₄₀, 40 Gy; and group A₄₅/B₄₅, 45 Gy. The left mandibular molars of the rats were extracted 1 week after irradiation. The rats were sacrificed 3 weeks after tooth extraction, and the mandible specimens were obtained for gross observation, micro-CT scanning, and histological detection to evaluate the success rate of modeling.

Results

At 3 weeks after dental extractions, complete gingival healing was found in the regions of dental extractions in groups A₃₅ and A₄₀. However, failed gingival healing and bone exposure were found in groups A₄₅ and B. Hematoxylin and eosin staining showed necrotic bone of the irradiated mandible in groups A₄₀, A₄₅,_{and B, with success modeling rates of 40% in group A and 93.3% in group B.}

Conclusion

Small-animal irradiator irradiation is an ideal device for establishing ORNJ model.

Key words: linear accelerator, small-animal irradiator, osteoradionecrosis of jaw, model

CLC Number:

R 782

Chen Huawei, Zhang Shengfu, He Haitao.. Effects of different irradiators on the establishment of osteoradionecrosis model of rat mandible[J]. West China Journal of Stomatology, 2021, 39(5): 524-530.

Figures/Tables 7

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Tab 1

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时间	CN组	A组			B组
时间	CN组	35 Gy	40 Gy	45 Gy	35 Gy	40 Gy	45 Gy
照射前	0.312 2±0.007 2	0.310 0±0.014 1	0.316 7±0.012 5	0.310 0±0.003 9	0.309 8±0.018 7	0.313 8±0.010 0	0.313 4±0.008 7
照射结束时	0.330 6±0.014 6	0.274 0±0.0114	0.273 0±0.013 1	0.274 8±0.021 3	0.318 0±0.022 5	0.322 7±0.009 7	0.314 5±0.010 7
照射后1周	0.370 8±0.012 0	0.237 0±0.015 1	0.226 7±0.037 7	0.201 0±0.017 2	0.342 7±0.032 9	0.324 3±0.056 2	0.288 3±0.016 4
拔牙后3周	0.442 6±0.033 2	0.346 7±0.006 2	0.314 0±0.006 5	0.265 0±0.022 5	0.443 3±0.059 5	0.437 3±0.046 3	0.406 3±0.041 1

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