Disheveled 2 around the implant at an early stage of hyperlipidemic rats

doi:10.7518/hxkq.2018.01.016

Abstract

Abstract:

Objective This study aims to investigate the expression of disheveled 2 (Dvl2) around the implant of hyperli-pidemic rats at an early stage after the implantation. Methods A total of 24 Wistar rats were divided equally into the experi-mental group fed with high-fat diet group and control group fed with a normal diet. After 8 weeks, the serum lipid levels were detected, and rats received implants in the femur metaphysis. Rats were sacrificed at 1, 3, and 5 days after implantation, and the bones around implants were obtained. Methylene blue-acid fuchsin staining was performed to observe the implant-bone interface. Real-time polymerase chain reaction was performed on runt-related transcription factor 2 (Runx2), cathepsin K (CatK), and Dvl2. Dvl2 Western blot or immunoprecipitation, phosphorylation, and ubiquitination were also conducted. Results In the experimental group, less osteoblasts, lower expression of Runx2 and Dvl2, and lower Dvl2 phosphorylation (P<0.05) than those of the control group were observed; furthermore, the CatK expression and Dvl2 ubiquitination were higher than those in the control group (P<0.05). Conclusion Hyperlipidemia may suppress bone remodeling around the implant at an early stage by Dvl2 down-regulation, phosphorylation, and up-regulated ubiquitination.

Key words: hyperlipidemia, bone remodeling, disheveled 2, Wnt signal pathway

CLC Number:

R783

Xiaofei Dong, Hui Wang, Jing Lan. Disheveled 2 around the implant at an early stage of hyperlipidemic rats[J]. West China Journal of Stomatology, 2018, 36(1): 82-86.

Figures/Tables 5

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基因名称	上游引物序列（5’—3’）	下游引物序列（5’—3’）
GAPDH	TGATGGGTGTGAACCACGAG	CCCTTCCACGATGCCAAAGT
Dvl2	ACCTCCACCATTACCCCCTT	CCTCCTAGGTCGGTCTCGTC
Runx2	CAGACACAATCCTCCCCACC	GCCAGAGGCAGAAGTCAGAG
CatK	TGGTTCCTGTTGGGCTTTCA	CCTTTGCCGTGGCGTTATAC

测量项目	对照组	实验组
TC	1.26±0.20	2.53±0.49*
TG	0.73±0.23	1.39±0.51*
HDL	0.88±0.18	1.14±0.23*
LDL	0.25±0.07	0.44±0.12*