华西口腔医学杂志 ›› 2018, Vol. 36 ›› Issue (4): 398-403.doi: 10.7518/hxkq.2018.04.010

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基质细胞衍生因子1通过整合素αvβ3-CXC族趋化因子受体4和7诱导头颈部鳞状细胞癌转移的研究

李鹏1(), 李文鹿2, 齐金星1   

  1. 1.郑州大学附属肿瘤医院头颈甲状腺科,郑州 450008
    2.郑州大学第一附属医院口腔颌面外科,郑州 450052
  • 收稿日期:2017-09-09 修回日期:2018-05-20 出版日期:2018-08-01 发布日期:2018-08-01
  • 作者简介:

    李鹏,主治医师,博士,E-mail:lipeng1528@163.com

  • 基金资助:
    国家自然科学基金青年科学基金(81402578,81703298)

Stromal cell-derived factor-1 inducing metastasis squamous cell carcinoma of head and neck induced via integrin αvβ3-CXC chemokine receptor 4/7 biological axis

Peng Li1(), Wenlu Li2, Jinxing Qi1   

  1. 1. Dept. of Head Neck and Thyroid, Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou 450008, China
    2. Dept. of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
  • Received:2017-09-09 Revised:2018-05-20 Online:2018-08-01 Published:2018-08-01
  • Supported by:
    The National Natural Science Foundation of China Youth Fund (81402578, 81703298).

摘要:

目的 观察基质细胞衍生因子1(SDF-1)诱导、LM609/AMD3100/CCX754抑制头颈部鳞状细胞癌(SCCHN)细胞系PCI-13细胞迁移、细胞骨架以及对整合素(integrin)αvβ3表达的影响。方法 用Transwell迁移实验法、免疫荧光法和流式细胞仪分别观察SDF-1、LM609、AMD3100、CCX754对PCI-13细胞迁移、细胞骨架以及细胞内integrin αvβ3蛋白表达的影响。结果 SDF-1能增加细胞迁移、PCI-13细胞中integrin αvβ3蛋白的表达以及细胞伪足的产生,LM609、AMD3100、CCX754能明显降低SDF-1对细胞迁移和PCI-13的正向诱导作用。结论 SDF-1可以通过integrin αvβ3-CXC族趋化因子受体4和7生物轴诱导SCCHN的转移作用,LM609、AMD3100、CCX754能降低SDF-1对SCCHN细胞的活性调控作用。

关键词: 基质细胞衍生因子1, CXC族趋化因子受体, 头颈部鳞状细胞癌

Abstract:

Objective To observe the influence of LM609/AMD3100/CCX754 on chemotactic capability, cytoskeleton, and expression of integrin αvβ3 protein of squamous cell carcinoma of head and neck (SCCHN) cell line PCI-13 induced by stromal cell-derived factor-1 (SDF-1) in vitro. Methods Migration assays, flow cytometry and immunofluorescence were used to observe the effects of SDF-1, LM609, AMD3100 and CCX754 on the migration, cytoskeleton and the expression of integrin αvβ3 protein in PCI-13 cell lines. Results SDF-1 favored PCI-13 cell migration, pseudopod formation, and activities of integrin αvβ3 phosphorylation. LM609, AMD3100, and CCX754 blocked all these effects. Conclusion SDF-1 can induce metastatic SCCHN by integrin αvβ3-CXC chemokine receptor (CXCR) 4/CXCR7 axi. LM609, AMD3100, and CCX754 and can reduce the regulation of SDF-1 on SCCHN activity.

Key words: stromal cell-derived factor-1, CXC chemokine receptor, squamous cell carcinoma of head and neck

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