磷脂酶C-γ1tyr783在下颌功能前伸大鼠髁突软骨后部表达变化的研究

doi:10.7518/hxkq.2014.05.007

华西口腔医学杂志

磷脂酶C-γ1tyr783在下颌功能前伸大鼠髁突软骨后部表达变化的研究

盛海莹左艳萍陈潇苑学微

河北医科大学第一医院口腔正畸科，石家庄 050031

出版日期:2014-10-01 发布日期:2014-10-01
通讯作者: 左艳萍，教授，博士，E-mail：zyp6381@sina.com
作者简介:盛海莹，住院医师，硕士，E-mail：haiying198596@163.com，现工作于北京市通州区新华医院正畸科

Changes in expression of phospholipase C-gamma1tyr783 in young rat condylar cartilage during functional mandibular protraction

Sheng Haiying, Zuo Yanping, Chen Xiao, Yuan Xuewei.

Dept. of Orthodontics, The First Hospital of Hebei Medical University, Shijiazhuang 050031, China

Online:2014-10-01 Published:2014-10-01

摘要/Abstract

摘要：

目的研究大鼠下颌功能前伸后髁突软骨中磷脂酶C-γ1 tyr783（PLC-γ1 tyr783）表达及其变化规律，探讨 PLC-γ1tyr783在功能矫治前伸大鼠下颌髁突软骨骨改建中的作用，为临床上骨骼矫形治疗提供实验依据。方法选取4周龄健康雄性Sprague-Dawley（SD）大鼠60只，适应性饲养1周后随机等量分为实验组和对照组，实验组每天24 h佩戴自制下颌前伸斜面导板矫治器，对照组不戴矫治器。2组大鼠分别于戴用矫治器1、3、7、14、21、28 d时各断颈处死5只，取双侧髁突，常规固定、脱钙、脱水、包埋。石蜡切片采用免疫组织化学方法结合图像分析检测PLC-γ1tyr783在髁突软骨中的表达分布及其变化规律。结果对照组PLC-γ1tyr783表达水平随着实验周期的推移逐渐减弱（P>0.05），实验组大鼠髁突软骨处PLC-γ1tyr783的表达在第14天时达到高峰，与对照组相比差异有统计学意义（P<0.01）。结论 PLC-γ1tyr783参与了下颌功能前伸大鼠髁突软骨的骨改建。

关键词: 下颌功能前伸, 髁突软骨, 磷脂酶C-&gamma, 1 tyr783, 骨改建

Abstract:

Objective To investigate the changes in the expression of phospholipase C-gammaltyr783 (PLC-γ1tyr783) in the condylar cartilage of a young rat during functional mandibular protraction. This work also explores the function of PLC-γ1tyr783 in the rat mandibular condylar cartilage bone remodeling, which could provide experimental evidence for clinical bone orthopedic work. Methods A total of 60 four-week-old male Sprague–Dawley (SD) rats were used in this study. The rats were divided equally and randomly into experimental group and control group. The functional appliances that were fitted to the upper incisors of the animals in the experimental group were worn 24 h a day after the rats were fed for 7 d with homemade pellet feed. The animals in the experimental group, along with their matched controls, were sacrificed after 1, 3, 7, 14, 21, and 28 d. The bilateral condylar was fixed, decalcified, dehyded, and then conventional paraffin embedded. Immunohistochemistry of PLC-γ1tyr783 was applied to observe its express distribution and variation. Results The expression of PLC-γ1tyr783 decreased gradually in the control group, which showed age-related changes (P>0.05). On the 14th day, PLC-γ1tyr783 expression in the experimental group was significantly higher than that in the control group. PLC-γ1tyr783 expression began to appear statistically and significantly different between the two groups (P<0.01). Conclusion PLC-γ1tyr783 is involved in the bone remodeling process of the rat condylar cartilage after functional mandibular protraction.

Key words: functional mandibular protraction, condylar cartilage, phospholipase C-gammaltyr783, bone remodeling

盛海莹左艳萍陈潇苑学微. 磷脂酶C-γ1tyr783在下颌功能前伸大鼠髁突软骨后部表达变化的研究[J]. 华西口腔医学杂志, doi: 10.7518/hxkq.2014.05.007.

Sheng Haiying, Zuo Yanping, Chen Xiao, Yuan Xuewei.. Changes in expression of phospholipase C-gamma1tyr783 in young rat condylar cartilage during functional mandibular protraction[J]. West China Journal of Stomatology, doi: 10.7518/hxkq.2014.05.007.

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磷脂酶C-γ1tyr783在下颌功能前伸大鼠髁突软骨后部表达变化的研究

Changes in expression of phospholipase C-gamma1tyr783 in young rat condylar cartilage during functional mandibular protraction

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