华西口腔医学杂志 ›› 2021, Vol. 39 ›› Issue (3): 279-285.doi: 10.7518/hxkq.2021.03.006

• 基础研究 • 上一篇    

不同种植体表面性质对雪旺细胞生物学行为影响的研究

王艳颖1,2(), 宫苹3, 张健1,2()   

  1. 1.天津市口腔医院(南开大学口腔医院)种植科,天津 300041
    2.天津市口腔功能重建重点实验室,天津 300041
    3.口腔疾病研究国家重点实验室 国家口腔疾病临床医学研究中心 四川大学华西口腔医院种植科,成都 610041
  • 收稿日期:2020-06-15 修回日期:2021-03-10 出版日期:2021-06-01 发布日期:2021-05-26
  • 通讯作者: 张健 E-mail:24548356@qq.com;zhangstoma@hotmail.com
  • 作者简介:王艳颖,副主任医师,博士,E-mail:24548356@qq.com
  • 基金资助:
    国家自然科学基金(81901062);天津市自然科学基金青年项目(19JCQNJC13300);天津市临床医学重点学科(口腔医学-口腔颌面外科学)专项基金项目(HWZX010)

Effects of different implant surface properties on the biological behavior of Schwann cells

Wang Yanying1,2(), Gong Ping3, Zhang Jian1,2()   

  1. 1.Dept. of Implantation, Tianjin Stomatological Hospital (Hospital of Stomatology, Nankai University), Tianjin 300041, China
    2.Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, Tianjin 300041, China
    3.State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Implantation, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
  • Received:2020-06-15 Revised:2021-03-10 Online:2021-06-01 Published:2021-05-26
  • Contact: Zhang Jian E-mail:24548356@qq.com;zhangstoma@hotmail.com
  • Supported by:
    The National Natural Science Foundation of China(81901062);Natural Science Foundation of Tianjin(19JCQNJC13300);Tianjin Key Discipline Foundation of Clinical Medicine(HWZX010)

摘要: 目的

探讨不同种植体表面性质对于雪旺细胞(SCs)生物学行为的影响。

方法

将SCs接种于3种种植体表面,分别是光滑表面(SMO)、大颗粒喷砂酸蚀表面(SLA)和亲水性化学活化大颗粒喷砂酸蚀表面(modSLA)。接种不同时间后,采用扫描电子显微镜观察钛片表面的SCs形态和黏附,MTT法检测细胞增殖活性,酶联免疫吸附测定法检测SCs相关蛋白神经生长因子(NGF)和脑源性神经营养因子(BDNF)的表达,实时荧光定量聚合酶链反应(PCR)检测NGF和BDNF mRNA表达水平的变化。

结果

SCs在3种钛片表面均良好黏附伸展和增殖。接种第3、5、7天时,SMO组的OD值均高于SLA组和modSLA组,差异有统计学意义(P<0.05)。接种第3天时,SLA组、modSLA组的NGF、BDNF及其mRNA的表达均高于SMO组(P<0.05),mod-SLA组高于SLA组(P<0.05)。

结论

不同种植体表面性质对SCs生物学行为的影响具有差异,光滑表面有利于SCs的增殖,而modSLA表面可促进SCs早期分泌神经营养因子以及其基因表达。

关键词: 种植体, 表面处理, 雪旺细胞, 神经再生

Abstract: Objective

This study investigated the effects of different implant surface properties on the biological behavior of Schwann cells.

Methods

Schwann cells (SCs) were cultured on three types of implant surfaces including smooth polished (SMO), sand-blasted, large grit, acid-etched (SLA), and chemically-modified SLA (modSLA). At different time points, the morphology and adhesion of SCs on the implant surfaces were observed by scanning electron microscope. Cell proliferation activity was detected by MTT method. The expression levels of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) were detected by enzyme-linked immunosorbent assay. Changes in the mRNA levels of NGF and BDNF were detected by real-time fluorescent quantitative polymerase chain reaction (PCR).

Results

SCs adhered, stretched, and proliferated well on the three types of implant surfaces. On the 3rd, 5th, and 7th days, the OD values of the SMO group were higher than those of the SLA group and the modSLA group, and the difference was statistically significant (P<0.05). On the 3rd day, the expression and mRNA levels of NGF and BDNF in the SLA group and the modSLA group were higher than those in the SMO group (P<0.05); in particular, the levels in the modSLA group were higher than those in the SLA group (P<0.05).

Conclusion

Different implant surface properties have different effects on the biological behavior of SCs. Proliferation of SCs is significantly promoted by smooth surface, while secretion and gene expression of neurotrophic factors are significantly promoted by modSLA surface at early stage.

Key words: implant, surface treatment, Schwann cells, nerve regeneration

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