华西口腔医学杂志 ›› 2020, Vol. 38 ›› Issue (3): 240-244.doi: 10.7518/hxkq.2020.03.002

• 基础研究 • 上一篇    下一篇

血管基质组分与软骨细胞共培养促进体内软骨再生

巴凯1, 倪端2, 王新波1, 魏雪琴1, 李娜1, 郑廉1()   

  1. 1.郑州大学第一附属医院口腔医学中心,郑州 450000
    2.武警河南总队医院急诊医学科,郑州 450000
  • 收稿日期:2019-05-15 修回日期:2020-03-13 出版日期:2020-06-01 发布日期:2020-05-28
  • 通讯作者: 郑廉 E-mail:Zhenglian0726@163.com
  • 作者简介:巴凯,主治医师,博士,E-mail:k.ba@msn.com
  • 基金资助:
    国家自然科学基金(81500826);郑州大学第一附属医院青年基金

Chondrocyte cocultures with stromal vascular fraction of adipose tissue promote cartilage regeneration in vivo

Ba Kai1, Ni Duan2, Wang Xinbo1, Wei Xueqin1, Li Na1, Zheng Lian1()   

  1. 1.Dept. of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China
    2.Dept. of Emergency Medicine, Armed Police Corps Hospital of Henan, Zhengzhou 450000, China
  • Received:2019-05-15 Revised:2020-03-13 Online:2020-06-01 Published:2020-05-28
  • Contact: Lian Zheng E-mail:Zhenglian0726@163.com
  • Supported by:
    Supported by: The National Natural Science Foundation of China(81500826);The Youth Foundation of the First Affiliated Hospital of Zhengzhou University

摘要:

目的 软骨损伤后自我修复能力有限,本研究通过对比血管基质组分(SVF)和脂肪间充质干细胞(ASCs)在体内促进软骨细胞成软骨能力的差异,为软骨再生筛选更加优秀的种子细胞。方法 本研究首先构建聚-β-羟基丁酸(PHB)/3-羟基丁酸与3-羟基己酸共聚酯(PHBHHx)三维生物支架材料,然后分离培养兔脂肪/软骨细胞,检测培养于支架上的细胞的形态和增殖,最后建立兔膝关节软骨缺损修复模型,将SVF和ASCs分别与软骨细胞共培养于支架上,植入缺损部位,10周后对比评估原位软骨缺损修复的效果。结果 接种在支架上的细胞在体外表现出更好的黏附和增殖。与软骨细胞共培养,脂肪源性SVF比ASCs更能促进体内软骨组织的生成。结论 PHB/PHBHHx共混体系是一种优秀的软骨组织工程材料。新鲜的SVF比体外扩增培养的ASCs更能促进软骨细胞生成软骨基质。SVF有望成为一种更好的软骨修复的细胞来源。

关键词: 软骨细胞, 血管基质组分, 共培养, 软骨再生

Abstract:

Objective This study aimed to compare the cartilage regeneration of the stromal vascular fraction (SVF) cells and adipose-derived mesenchymal stem cells (ASCs) cocultured with chondrocytes seeded on the scaffolds. Methods The cellular morphologies and proliferation capabilities on the scaffolds were evaluated. The scaffolds with the coculture of ASCs/SVF and chondrocytes were implanted into the full thickness cartilage defective rabbit joints for 10 weeks. Results The cells seeded into the scaffolds showed good adhesion and proliferation. Implantation with SVF and chondrocytes revealed desirable in vitro healing outcomes. Conclusion The SVF cells were better than ASCs in terms of the formation of cartilage matrix in a coimplantation model. Without in vitro expansion, the SVF cells are good cell sources for cartilage repair.

Key words: chondrocytes, stromal vascular fraction, cocultures, cartilage regeneration

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