华西口腔医学杂志

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口腔扁平苔藓患者血清中白细胞介素-12和白细胞介素-27表达与免疫功能的相关性

黄韵颖 周塑 蔡扬   

  1. 贵州医科大学附属医院口腔内科,贵阳 550004
  • 收稿日期:2015-07-21 修回日期:2016-01-18 出版日期:2016-04-01 发布日期:2016-04-01
  • 通讯作者: 蔡扬,教授,博士,E-mail:caiyang85@163.com
  • 作者简介:黄韵颖,硕士,E-mail:305014272@qq.com
  • 基金资助:

    国家自然科学基金(81460104)

Expression of interleukin-12 and interleukin-27 proteins and immune status in serum of patients with oral lichen planus

Huang Yunying, Zhou Su, Cai Yang.   

  1. Dept. of Stomatology, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China
  • Received:2015-07-21 Revised:2016-01-18 Online:2016-04-01 Published:2016-04-01
  • Contact: Cai Yang, E-mail: caiyang85@163.com.
  • Supported by:

    Natural Science Foundation of China (81460104).

摘要:

目的 了解口腔扁平苔藓(OLP)患者血清中白细胞介素(IL)-12和IL-27的表达与患者免疫功能状况的相关性,探讨IL-12和IL-27在OLP免疫发病机制中的作用及意义。方法  选取对照组健康者20例和OLP组患者30例(其中网纹型15例,糜烂型15例),通过流式细胞术分析OLP患者外周血淋巴细胞亚群CD3+、CD4+、CD8+、CD19+、CD16+56[自然杀伤细胞(NK)]的表达情况,散射比浊法检测患者体液免疫指标免疫球蛋白(Ig)G、IgA、IgM、C3、C4的表达情况。采用酶联免疫吸附试验(ELISA)方法检测血清中IL-12和IL-27的表达水平,分析IL-12和IL-27的表达与OLP患者免疫功能状况及临床特征的相关性。结果  与实验室标准值相比,OLP患者细胞免疫中CD3+、CD4+、CD8+水平降低,CD19+水平增高(P<0.05);体液免疫中IgM水平增高,C4水平降低(P<0.05)。OLP患者血清中IL-12与IL-27表达水平均高于对照组(P<0.05)。同时相关分析显示,OLP患者血清中IL-12和IL-27的表达水平呈正相关关系(r=0.912,P<0.01)。但是IL-12及IL-27表达水平与OLP体征计分、病程计分等临床特征无相关性(P>0.05)。OLP患者中IL-12和IL-27的表达与CD16+56(NK)存在负相关关系(r1=-0.416,P1=0.022;r2=-0.392,P2=0.032),与IgG存在正相关关系(r1=0.445,P1=0.014;r2=0.549,P2=0.002)。结论 OLP患者主要以细胞免疫功能低下为主,同时伴有一定程度的体液免疫功能的紊乱。IL-12和IL-27的异常高表达可能协同引起且促进了OLP的炎症发生发展,并且通过机体代偿性负反馈作用参与了对炎症性免疫应答的调节,在OLP的发病机制中起到了一定作用。

关键词: 口腔扁平苔藓, 白细胞介素-12, 白细胞介素-27, 免疫, 酶联免疫吸附试验

Abstract:

Objective This study aimed to conduct a preliminary study on the possible role and significance of interleukin (IL)-12 and IL-27 in the pathogeneses of oral lichen planus (OLP). Methods Thirty cases of patients with OLP (fifteen cases of reticular OLP and fifteen cases of erosive OLP) were enrolled in this study, and twenty cases of healthy people served as controls. Lymphocyte subsets CD3+, CD4+, CD8+, CD19+, and CD16+56 [natural killer cell (NK)] were tested using flow cytometry, and humoral immunity [immunoglobulin (Ig)G, IgA, IgM, C3, C4] were examined using nephelometry assays. IL-12 and IL-27 contents in serum of patients with OLP and normal controls were detected through enzyme linked immunosorbent assay. The correlations between the levels of IL-12, IL-27, immune status, and clinical characteristics of patients with OLP were analyzed, respectively. Results CD3+, CD4+, and CD8+ in patients with OLP were markedly lower than the normal value, whereas CD19+ of OLP in patients was significantly higher than the normal value (P<0.05). IgM in patients with OLP was increased, whereas C4 was declined (P<0.05). IL-12 and IL-27 levels showed significant upregulation of OLP patients compared with control groups (P<0.05). Meanwhile, positive correlations existed between IL-12 and IL-27 levels in the serum of patients with OLP (r=0.912, P<0.01). No significant correlations of IL-12 and IL-27 expressions with clinical characteristics of OLP were found (P>0.05). Negative correlations of IL-12 and IL-27 levels with CD16+56(NK) cells were observed (r1=-0.416, P1=0.022; r2=-0.392, P2=0.032, respectively), whereas a positive correlation existed for IgG (r1=0.445, P1=0.014; r2=0.549, P2=0.002, respectively). Conclusion  A cellular immune dysfunction mainly dominate in patients with OLP, accompanied by some degree of humoral-immunity-function disorder. The abnormally high expressions of IL-12 and IL-27 are possibly synergized and promoted inflammation development in OLP. Its promotion takes place through the negative feedback regulation of humoral immune responses, which are involved in the regulation of immune mechanisms of OLP.

Key words: oral lichen planus, interleukin-12, interleukin-27, immune, enzyme linked immunosorbent assay