华西口腔医学杂志

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角质细胞生长因子对口腔黏膜上皮细胞凋亡的作用研究

魏美荣1,2 李国菊1 张达3 颜世果1 戚向敏1   

  1. 1.山东大学口腔医院综合科，山东省口腔生物医学重点实验室，济南 250012；2.潍坊市人民医院口腔科，潍坊 261041；3. 山东省千佛山医院口腔科，济南 250015
  • 出版日期:2013-12-01 发布日期:2013-12-01
  • 通讯作者: 戚向敏，Tel：0531-88382940
  • 作者简介:魏美荣（1987—），女，山东人，硕士
  • 基金资助:

    山东省自然科学基金面上资助项目（ZR2011HM027）

Study on the function of keratinocyte growth factor on apoptosis of oral mucosal epithelial cells

Wei Meirong1,2, Li Guoju1, Zhang Da3, Yan Shiguo1, Qi Xiangmin1.   

  1. 1. Dept. of General Diagnosis, School of Stomatology, Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China; 2. Dept. of Stomatology, Weifang People’s Hospital, Weifang 261041, China; 3. Dept. of Stomatology, Shandong Provincial Qianfoshan Hospital, Jinan 250015, China
  • Online:2013-12-01 Published:2013-12-01

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摘要：

目的 研究不同浓度角质细胞生长因子（KGF）对口腔黏膜上皮细胞凋亡的作用，为探讨KGF在口腔黏膜病发生发展中的作用提供依据。方法 将不同浓度的KGF（对照组0 ng·mL-1，实验1组5 ng·mL-1，实验2组25 ng·mL-1，实验3组50 ng·mL-1）分别加入体外培养的口腔黏膜上皮细胞，培养12、24、48 h后，倒置显微镜下观察其对细胞形态的影响，并用流式细胞仪检测细胞凋亡情况，荧光实时定量检测细胞凋亡相关基因Bcl-2、Bax mRNA的表达水平。结果 1）实验组较对照组细胞贴壁明显，且48 h时实验3组细胞核仁明显。2）培养48 h时，4组之间的细胞凋亡率、Bcl-2 mRNA、Bax mRNA表达均有统计学差异，随着KGF浓度的增加，细胞凋亡率和Bax mRNA表达逐渐降低，Bcl-2 mRNA表达逐渐升高（P<0.05）。结论 KGF可通过上调Bcl-2 mRNA和下调Bax mRNA的表达抑制上皮细胞的凋亡。

关键词: 角质细胞生长因子, 细胞凋亡, 口腔黏膜上皮细胞

Abstract:

Objective  To study the function of keratinocyte growth factor (KGF) on apoptosis of oral mucosal epithelial cells and to provide a basis for further investigation of the role of KGF in the occurrence and development of oral mucosal diseases. Methods  Different concentrations of KGF (control group, 0 ng·mL-1; experiment 1 group, 5 ng·mL-1; experimental 2 group, 25 ng·mL-1; experiment 3 group, 50 ng·mL-1) were added in oral mucosa epithelial cells cultured in vitro. After training for 12, 24, and 48 h, cell morphology was observed under an inverted microscope. Apoptosis was detected by using a flow cytometry instrument, and mRNA expression of apoptosis-related genes Bcl-2 and Bax was detected by using Real-Time fluo-rescent quantitative detection. Results  Cell adherence of the experimental group was more obvious than that of the control group, and the cell nucleolus of the experiment 3 group was obviously cultured at 48 h. After culturing for 48 h, the apoptosis rate and Bcl-2 and Bax mRNA expression among the four groups were statistically significant. The increase of KGF concen-tration, apoptosis rate, and Bax mRNA expression gradually reduced, whereas Bcl-2 mRNA expression increased (P<0.05). Conclusion  KGF may inhibit epithelial cell apoptosis through upregulation of Bcl-2 mRNA and downregulation of Bax mRNA.

Key words: keratinocyte growth factor, apoptosis, oral mucosal epithelial cells

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