关键词: 金属蛋白酶组织抑制剂, 涎腺腺样囊性癌, β-肌营养不良蛋白聚糖

Abstract:

Objective  To investigate the expression of β-dystroglycan (β-DG) and the roles of tissue inhibitor of metalloproteinases (TIMPs) on β-DG in salivary adenoid cystic carcinoma (SACC) . Methods  β-DG in highly lung metastatic cell line ACC-M and lowly lung metastatic one ACC-2 was tested by immunocytochemistry with different concentrations (10, 15, 20, 25 μmol ? L-1) of TIMPs, and that without the regulation of TIMPs was served as controls. β-DG was detected in seven specimens of SACC and ten cases of normal salivary gland tissues which were considered as a comparison group by immunohistochemistry. Results  There was no positive β-DG immune-staining at the ACC-2 and ACC-M cell lines without TIMPs in the cell culture. β-DG expressed after the regulation of TIMPs. β-DG expression was localized predominantly in basement membrane of the acinus, while the negative results were distributed in the carcinoma cells and around the cancer cell nests. Conclusion  β-DG is widely expressed by transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton, the fracture of this structure means that it is easy to invade and transfer, so restoration of β-DG expression by TIMPs is considered to be critical for successful treatment of SACC.

Key words: tissue inhibitor of metalloproteinases, salivary adenoid cystic carcinoma, β-dystroglycan