华西口腔医学杂志

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含铜不锈钢对血管内皮细胞黏附、增殖及凋亡的影响

徐璐1 张扬1 杨柯2 任玲2 王强3   

  1. 1.中国医科大学附属口腔医院  正畸科， 沈阳 110002； 2.中国科学院金属研究所 专用材料与器件研究部， 沈阳 110016；
    3.中国医科大学附属口腔医院  口腔材料学教研室， 沈阳 110002
  • 出版日期:2013-02-01 发布日期:2013-02-01
  • 通讯作者: 张扬，Tel：024-22891195
  • 作者简介:徐璐（1986—），女，辽宁人，硕士
  • 基金资助:

    国家自然科学基金资助项目（51171186）；辽宁省科技计划基金资助项目（2010225025）

Influence of copper -bearing stainless steel on adhesion, proliferation and apoptosis of vascular endothelial cell

Xu Lu1, Zhang Yang1, Yang Ke2, Ren Ling2, Wang Qiang3   

  1. 1. Dept. of Orthodontics, Stomatology School of China Medical University, Shenyang 110002, China; 2. Dept. of Specialized Materials and Devices, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China; 3. Dept. of Dental Materials, Stomatology School of China Medical University, Shenyang 110002, China
  • Online:2013-02-01 Published:2013-02-01

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摘要：

目的  观察含铜不锈钢对血管内皮细胞黏附、增殖及凋亡的影响，并与316L不锈钢进行比较。方法  将血管内皮细胞接种于两种材料表面，在培养1、2、3 d后，应用吖啶橙染色及甲基噻唑基四唑（MTT）法检测细胞在 两种材料表面黏附和增殖活性。并制备两种材料的浸提液，用浸提液培养血管内皮细胞，采用流式细胞术检测细 胞凋亡率。结果  荧光显微镜下，细胞在两种材料表面均伸展良好，呈铺路石状生长。在培养1、2 d时，含铜不锈 钢组黏附的细胞数多于316L不锈钢组（P<0.05）；3 d时两组间差异无统计学意义（P>0.05）。MTT结果显示，含铜不锈 钢组在1、2 d的吸光度值高于316L不锈钢组，差异有统计学意义（P<0.05）；而在培养3 d后，两组间差异无统计学意 义（P>0.05）。316L不锈钢组的早期凋亡率高于含铜不锈钢组，差异有统计学意义（P<0.05）。结论  含铜不锈钢较 316L不锈钢更利于血管内皮细胞的黏附及增殖，并可以降低血管内皮细胞的早期凋亡率。

关键词: 含铜不锈钢, 血管内皮细胞, 黏附, 增殖, 细胞凋亡

Abstract:

 Objective  To observe the influence of copper -bearing stainless steel on adhesion, proliferation and apoptosis of vascular endothelial cell（VEC）. Methods  The samples of two kinds of materials were inoculated with VEC and incubated for 1, 2 and 3 d. After stained by acridine orange, the cells adhered on the surface of samples were observed under a fluorescent microscope. The proliferation of VEC was detected by methyl thiazolyl tetrazolium（MTT） test. Next, leaching liquor of these two kinds of materials was prepared and used to culture VEC. The apoptosis of VEC was measured by flow cytometry. Results Fluorescent microscope showed VEC spread into fusiform shape, copper-bearing stainless steel surfaces had a significantly higher number of adherent VEC than 316L stainless steel at 1 and 2 d（P<0.05）. No significant difference of adherent VEC was observed between two kinds of materials at 3 d（P>0.05）. MTT results indicated that the optical density（OD） value of copper-bearing stainless steel were higher than that of 316L stainless steel at 1 and 2 d（P<0.05）. No significant difference of OD value was observed between two kinds of materials at 3 d（P>0.05）. The early apoptosis ratio of 316L stainless steel was higher than copper-bearing stainless stee（l P<0.05）. Conclusion  Copper-bearing stainless steel could promote the adhesion, proliferation of VEC, and inhibit the early apoptosis ratio of VEC.

Key words: copper-bearing stainless steel, vascular endothelial cell, adhesion, proliferation, apoptosis

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