磷脂酰肌醇-3-激酶/蛋白质丝氨酸-苏氨酸激酶信号通路与正畸牙移动的关系

doi:10.3969/j.issn.1000-1182.2011.03.006

华西口腔医学杂志

磷脂酰肌醇-3-激酶/蛋白质丝氨酸-苏氨酸激酶信号通路与正畸牙移动的关系

刘奕王岩孙素芬

中国医科大学口腔医院奉天门诊，沈阳110013

收稿日期:2011-06-25 修回日期:2011-06-25 出版日期:2011-06-20 发布日期:2011-06-20
通讯作者: 刘奕，Tel：024-31973999
作者简介:刘奕（1964—），女，辽宁人，教授，博士
基金资助:
辽宁省科学技术计划基金资助项目（2009225010-27）；沈阳市科学技术计划基金资助项目（1072033-1-00）

The relationship between phosphatidylinositol -3 -kinases/protein -serine -threonine kinase signaling pathway and orthodontic tooth movement

LIU Yi, WANG Yan, SUN Su-fen

Fengtian Dental Clinic, School of Stomatology, China Medical University, Shenyang 110013，China

Received:2011-06-25 Revised:2011-06-25 Online:2011-06-20 Published:2011-06-20
Contact: LIU Yi，Tel：024-31973999

摘要/Abstract

摘要：

目的探讨磷脂酰肌醇-3-激酶（PI3K）/蛋白质丝氨酸-苏氨酸激酶（AKt）信号通路与正畸牙移动的关系。方法选用24只日本大耳白兔建立正畸牙移动的动物模型，将实验动物上颌右侧戴矫治器，作为实验侧；上颌左侧未戴矫治器，作为对照侧。分别在戴矫治器3、5、7、14 d后各处死6只实验动物。用实时荧光定量聚合酶链反应（RQ-PCR）及Western blot免疫印迹分析方法对PI3K、AKt表达的变化进行检测。结果RQ-PCR结果显示：加力3 d后，牙周组织中PI3K、AKt mRNA表达增强；7 d后牙周组织中PI3K、AKt mRNA明显增强，随后缓慢下降，与对照侧相比，其差异有统计学意义（P＜0.05）。Western blot免疫印迹分析与RQ-PCR结果一致。结论正畸力作用下兔牙周组织中PI3K、AKt的表达增加。提示PI3K/AKt信号通路与正畸牙移动有关，PI3K/AKt信号通路参与正畸牙移动过程中的牙周组织改建。

关键词: 磷脂酰肌醇-3-激酶, 蛋白质丝氨酸-苏氨酸激酶, 实时荧光定量聚合酶链反应

Abstract:

Objective To study the relationship between phosphatidylinositol-3-kinases（PI3K）/protein-serine-threonine kinase（AKt） signaling pathway and orthodontic tooth movement. Methods Twenty-four rabbits were chosen to establish rabbit models for the study. The right maxillary teeth of each animal treated by orthodontics were as the test side, and the untreated left teeth were as the control side. The animals were sacrificed at 3, 5, 7, 14 d, respectively. The prepared tissue specimens were processed for the study. The changes of the expression of PI3K, AKt in periodontal tissues were detected by real-time quantitative-polymerase chain reaction（RQ-PCR）and Western blot techniques. Results RQ-PCR showed that the expression of PI3K, AKt mRNA dramatically changed at 3 d. The expression of PI3K, AKt mRNA in the test side was higher than the control side, especially at 7 d, and then decreased. Compared with the control side, there was statistical significant difference in the test side（P＜0.05）. The study obtained consistent conclusion from Western blot and RQ-PCR. Conclusion Expression of PI3K, AKt in rabbit periodontal tissues increase during orthodontic tooth movement, which prompts that PI3K/AKt signal pathways relate to orthodontic tooth movement and PI3K/AKt signal pathway involve in the periodontal tissue remodeling.

Key words: phosphatidylinositol-3-kinases, protein-serine-threonine kinase, real-time quantitative-polymerase chain reaction

刘奕王岩孙素芬. 磷脂酰肌醇-3-激酶/蛋白质丝氨酸-苏氨酸激酶信号通路与正畸牙移动的关系[J]. 华西口腔医学杂志, doi: 10.3969/j.issn.1000-1182.2011.03.006.

LIU Yi, WANG Yan, SUN Su-fen. The relationship between phosphatidylinositol -3 -kinases/protein -serine -threonine kinase signaling pathway and orthodontic tooth movement[J]. West China Journal of Stomatology, doi: 10.3969/j.issn.1000-1182.2011.03.006.

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磷脂酰肌醇-3-激酶/蛋白质丝氨酸-苏氨酸激酶信号通路与正畸牙移动的关系

The relationship between phosphatidylinositol -3 -kinases/protein -serine -threonine kinase signaling pathway and orthodontic tooth movement

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