Abstract:

Objective To investigate the effect of FAK on biological characteristics of tongue squamous cell carcinoma cell Tca8113. Methods Lipofect was used to transfect FAK into Tca8113 cell lines. The difference in expression of FAK between the transfected and non- transfected cell lines was detected by immunofluorescence assay. Then by use of transwell room and wash way, the difference in invasion and adhesion ability between the transfected and non- transfected cell lines was tested. MTT method was adopted in finding the change of proliferation. Results RT- PCR analysis showed that the FAK gene was downregulated by Tca8113 cells. FAK expression was significantly down regulated by the transfection of FAK ASODN（P<0.05）. Antisense FAK ODN significantly inhibited proliferation, adhesion and the invasion of Tca8113 cells. Conclusion FAK plays an important role on biological characteristics of tongue squamous cell carcinoma cell Tca8113.

Key words: focal adhesion kinase, tongue squamous cell carcinoma, transfection