Abstract:

The purpose of this study is to study the biological effects of recombinant human bone morphogenetic protein 2 (rhBMP2) on human periodontal ligament fibroblasts (HPDLFs) . Methods : Human periodontal ligament fibroblasts were primary cultured and detected the different doses of rhBMP2 on their proliferation , alkaline phosphatase (ALP) activity , osteocalcin (OC) synthesis and formation of the mineralized nodules. Results : rhBMP2 (0125～2 mgPml) had no remarkable effect on the prolifera2 tion of HPDLFs. The ALP activity , OC synthesis and formation of the mineralized nodules of HPDLFs were significantly stimulated by 015～2 mgPml rhBMP2. Conclusion : The effects of rhBMP2 on HPDLFs are dose- dependent. Not only can rhBMP2 stimulate the expression but also promote the maturation of osteoblastic phenotype of HPDLFs.

Key words: recombinant human bone morphogenetic protein 2 , human periodontal ligament fibroblasts , alkaline
phosphatase, osteocalcin , mineralization