Abstract:

Objective　To clone and sequence the upstream of mouse dentin sialophosphoprotein promoter.Methods　Genom- ic DNAwas obtained from Balb/c mouse blood. The upstream of mouse dentin sialophosphoprotein promoter segmentswas obtained by PCR. Then the segments were inserted into T-vector. The plasmids were identified by digestion with the restriction enzyme analysis. The positive clone was sequenced and compared with Genebank.Results　The upstream of mouse dentin sialophospho- protein promoter was divided into three sequences and three different target segments with 997 bp, 1 004 bp and 674 bp in length were obtained. After identified, sequenced and comparedwithGenebank, the sequences of the segmentswere consistentwiththose displayed on Genebank by 99%.Conclusion　The clone of the upstream of mouse dentin sialophosphoprotein promoterwas suc- cessful. This work will help to study the regulation of DSPP expression.

Key words: dentin sialophosphoprotein, polymerase chain reaction, promoter