Effect of glycosaminoglycans with different degrees of sulfation on chondrogenesis

doi:10.7518/hxkq.2023.2023055

Abstract

Abstract:

Objective This study aims to investigate the effects and mechanisms of chondroitin sulfate (CS), dermatan sulfate (DS), and heparin (HEP) on chondrogenesis of murine chondrogenic cell line (ATDC5) cells and the maintenance of murine articular cartilage in vitro. Methods ATDC5 and articular cartilage tissue explant were cultured in the medium containing different sulfated glycosaminoglycans. Cell proliferation, differentiation, cartilage formation, and mechanism were observed using cell proliferation assay, Alcian blue staining, real-time quantitative polymerase chain reaction (RT-qPCR), and Western blot, respectively. Results Results showed that HEP and DS primarily activated the bone morphogenetic protein (BMP) signal pathway, while CS primarily activated the protein kinase B (AKT) signal pathway, further promoted ATDC5 cell proliferation and matrix production, and increased Sox9, Col2a1, and Aggrecan expression. Conclusion This study investigated the differences and mechanisms of different sulfated glycosaminoglycans in chondrogenesis and cartilage homeostasis maintenance. HEP promotes cartilage formation and maintains the normal state of cartilage tissue in vitro, while CS plays a more effective role in the regeneration of damaged cartilage tissue.

Key words: chondrogenesis, cartilage repair, sulfated glycosaminoglycan, degree of sulfation

CLC Number:

Q 249

Zheng Wen, Cai Ming-xiang, Peng Huizhen, Liu Minyi, Liu Xiangning. Effect of glycosaminoglycans with different degrees of sulfation on chondrogenesis[J]. West China Journal of Stomatology, 2023, 41(4): 395-404.

Figures/Tables 5

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