Abstract:

Objective To assess suppression effects of vector-based small interfering RNA（siRNA） on vascular endothelial growth factor（VEGF） expression of human tongue squamous carcinoma cell line（Tca8113） in vitro. Methods Two siRNA targeting VEGF constructed in eukaryotic expression vector（Pu-VEGF-siRNA1, Pu-VEGFsiRNA2）,eukaryotic expression vector as the experiment control, all of which were transfected into Tca8113 cells with Lipofectamine 2000. Non-transfection cell was used as negative control. VEGF mRNA and protein were detected by reverse transcription polymerase chain reaction（RT-PCR）, immunohistochemistry and enzyme linked immunosorbent assay（ELISA）, respectively. Results Compared to the experimental and negative controls, the expression of VEGF mRNA and protein were significantly decreased in the Pu-VEGF-siRNA1 group and Pu-VEGF-siRNA2 group. But there were no significant differences between two controls（P >0.05）. Conclusion Vector -based siRNAs targeting VEGF are efficient in down-regulating VEGF expression in Tca8113 cells.

Key words: tongue cancer, vascular endothelial growth factor, small interfering RNA